半岛bd体育手机客户端 介绍
目录号 Serial |
TCHu145 |
标识符 Identifier |
CSTR:19375.09.3101HUMTCHu145 |
描述 Description |
IMR-32细胞是1967年四月 W.W. Nichols, J. Lee and S. Dwight从一位13个月大的男婴下腹部肿瘤中分离建株。 诊断认为是神经母细胞瘤,并有少部分区域的器质性分化。 包括两种类型的细胞。 主要的是小的神经母细胞样的细胞。 另一种是大的透明成纤维细胞。 已经证明细胞可以传代培养到80代以上。 在本库通过支原体检测。 在本库通过STR检测。 |
动物种别 Organism |
人 |
性别 Gender |
男 |
组织来源 Tissue and Cell Type |
神经母细胞瘤,大脑 |
形态 Morphology |
神经母细胞,成纤维细胞 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
REFERENCE |
Tumilowicz JJ ct al. Definition of a continuous human cellline derived from neuroblastoma. Cancer Res. 30:21 [0, 1970PubMed: 70293585Rostomily RC et ah Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997 PubMed: 97413638Maestrini E et ah A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Nath Acad. Sci. USA 93: 674-678, 1996 PubMed: 96149362Hay, R. J., CaputG J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223 -227, 1988.Fleming, D.O, Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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