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细胞培养基 >> 细胞株
Jurkat, Clone E6-1人白血病T淋巴细胞(2018年新引进)
Jurkat, Clone E6-1人白血病T淋巴细胞(2018年新引进)图片
半岛bd体育手机客户端 介绍
目录号
Serial
SCSP-513
标识符
Identifier
CSTR:19375.09.3101HUMSCSP513
描述
Description
人白血病T淋巴细胞,Jurkat细胞由Schneider建立,来源于一个14岁的男孩的外周血。该克隆是 Jurkat-FHCRC细胞株的一个克隆(Jurkat的一个衍生株),经佛波酯(phorbol esters)和外源凝集素(lectins)或抗T3单克隆抗体(需要两种物质共同诱导)诱导后可产生大量IL-2。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测结果无误。STR结果如下:D5S818: 9;D13S317: 8,12;D7S820: 8,12;D16S539: 11;vWA: 18;THO1: 6,9.3;Amelogenin: X,Y;TPOX: 8,10;CSF1PO: 11,12
动物种别
Organism
性别
Gender
男性
组织来源
Tissue and Cell Type
外周血
形态
Morphology
成淋巴细胞状,悬浮生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
1. 人白血病T淋巴细胞Jurkat, Clone E6-1完全培养液配方(100 ml)
RPMI 1640 Medium (Invitrogen, 11875-093)  88 ml
FBS (Gibco)  10 ml
Glutamax(invitrogen 35050-061)        1 ml
Sodium Pyruvate 100 mM Solution (invitrogen 11360070)  1 ml
参考传代周期:不超过300万细胞 /mL,建议将活细胞密度控制在10万-100万/mL之间
参考传代密度:10万活细胞/mL
参考换液频率:2-3天
冻存液:完全培养液95%,DMSO 5%
细胞状态:悬浮生长
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
2. 注意事项:
a) 该细胞为悬浮细胞,根据培养经验以及客户的反馈,传代时使用【半换液法】对细胞状态较为有利,因此我库建议您使用【半换液法】进行传代。同时,您在收到细胞后,请不要通过离心的方式收集细胞,可以直接向培养瓶中添加等体积的新鲜培养液,然后将细胞吹打均匀后移入两个新的T25培养瓶中继续培养即可。
b) 细胞对血清质量较为敏感,我库建议您使用进口大品牌优质血清进行培养。
c) 该细胞对细胞密度较为敏感,培养、传代时请注意保持细胞密度在合适的范围。
供应限制
Permits and Restrictions
A。仅供研究之用。
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