Leupeptin Hemisulfate(NK381) - 半岛电竞官方网址

Molecular Weight (MW)	475.59
Formula	C20H38N6O4.1/2H2SO4
CAS No.	103476-89-7
Storage	-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)	DMSO: 95 mg/mL (199.8 mM)
Water: 95 mg/mL (199.8 mM)
Ethanol: 95 mg/mL (199.8 mM)
SMILES Code	CC(C)C[C@@H](C(N)=O)N(C([C@H](CC(C)C)NC(C)=O)=O)[C@H](C=O)CCCNC(N)=N.CC(C)C[C@@H](C(N)=O)N(C([C@H](CC(C)C)NC(C)=O)=O)[C@H](C=O)CCCNC(N)=N.O=S(O)(O)=O
Synonyms	NK-381; NK 381; Leupeptin hemisulfate; NK381; Chemical Name: (S)-2-acetamido-N-((S)-1-amino-4-methyl-1-oxopentan-2-yl)-N-((S)-5-guanidino-1-oxopentan-2-yl)-4-methylpentanamide hemisulfate InChi Key: HFKRKHUDDFQRJB-VFFZMTJFSA-N InChi Code: InChI=1S/2C20H38N6O4.H2O4S/c21-12(2)9-16(25-14(5)28)19(30)26(17(18(21)29)10-13(3)4)15(11-27)7-6-8-24-20(22)23;1-5(2,3)4/h211-13,15-17H,6-10H2,1-5H3,(H2,21,29)(H,25,28)(H4,22,23,24);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1

Molecular Weight (MW)

475.59

Formula

C20H38N6O4.1/2H2SO4

CAS No.

103476-89-7

Storage

-20℃ for 3 years in powder form

-80℃ for 2 years in solvent

Solubility (In vitro)

DMSO: 95 mg/mL (199.8 mM)

Water: 95 mg/mL (199.8 mM)

Ethanol: 95 mg/mL (199.8 mM)

SMILES Code

CC(C)C[C@@H](C(N)=O)N(C([C@H](CC(C)C)NC(C)=O)=O)[C@H](C=O)CCCNC(N)=N.CC(C)C[C@@H](C(N)=O)N(C([C@H](CC(C)C)NC(C)=O)=O)[C@H](C=O)CCCNC(N)=N.O=S(O)(O)=O

Synonyms

NK-381; NK 381; Leupeptin hemisulfate; NK381;

Chemical Name: (S)-2-acetamido-N-((S)-1-amino-4-methyl-1-oxopentan-2-yl)-N-((S)-5-guanidino-1-oxopentan-2-yl)-4-methylpentanamide hemisulfate

InChi Key: HFKRKHUDDFQRJB-VFFZMTJFSA-N

InChi Code: InChI=1S/2C20H38N6O4.H2O4S/c2*1-12(2)9-16(25-14(5)28)19(30)26(17(18(21)29)10-13(3)4)15(11-27)7-6-8-24-20(22)23;1-5(2,3)4/h2*11-13,15-17H,6-10H2,1-5H3,(H2,21,29)(H,25,28)(H4,22,23,24);(H2,1,2,3,4)/t2*15-,16-,17-;/m00./s1

In Vitro	In vitro activity: Leupeptin, produced by various species of actinomycetes, strongly inhibit proteolysis. Leupeptin hemisulfate protects tubulin from endogenous proteolytic activities during the isolation procedure and results in increased tubulin purity. Leupeptin hemisulfate could restore up to 50% of hepatitis B surface antigen (HBsAg) expression in cell suspension cultures. Cell Assay: In cultures of MRC-C cells, Leupeptin prevented multiplication of the human coronavirus strain 229E. The IC50 value of Leupeptin in plaque tests was 0.4 μg/mL, whilst growth of host cells was unaffected by Leupeptin at 50 μg/mL. In single-cycle growth experiments, Leupeptin (100 μg/mL) reduced virus yield only if added within 2 hrs of infection, indicating its action on an early stage of virus replication.
In Vivo	Leupeptin was well tolerated by the animals and dose-dependently produced a substantial increase in LC3b-II in both the total tissue extracts and the lysosome enriched fraction (LE fraction). At the electron microscopy (EM) level, leupeptin induced the accumulation of electron-dense vesicular structures that, in hepatocytes, were visible by 60 min after treatment (40 mg/kg). The results suggested that Leupeptin enhanced LC3b-II levels in vivo by protecting this protein from being degraded inside lysosomes, and thus the leupeptin-based assay could be potentially used for studying the dynamics of macroautophagy in mice.
Animal model	C57BL/6NCrl male mice
Formulation & Dosage	0, 9, 18 36 and 40 mg/kg; i.p. injection
References	J Antibiot (Tokyo). 1969 Nov;22(11):558-68; Cell Biol Int Rep. 1985 Sep;9(9):849-57; Plant Cell Rep. 2007 Sep;26(9):1575-84.

In Vitro

In vitro activity: Leupeptin, produced by various species of actinomycetes, strongly inhibit proteolysis. Leupeptin hemisulfate protects tubulin from endogenous proteolytic activities during the isolation procedure and results in increased tubulin purity. Leupeptin hemisulfate could restore up to 50% of hepatitis B surface antigen (HBsAg) expression in cell suspension cultures.

Cell Assay: In cultures of MRC-C cells, Leupeptin prevented multiplication of the human coronavirus strain 229E. The IC50 value of Leupeptin in plaque tests was 0.4 μg/mL, whilst growth of host cells was unaffected by Leupeptin at 50 μg/mL. In single-cycle growth experiments, Leupeptin (100 μg/mL) reduced virus yield only if added within 2 hrs of infection, indicating its action on an early stage of virus replication.

In Vivo

Leupeptin was well tolerated by the animals and dose-dependently produced a substantial increase in LC3b-II in both the total tissue extracts and the lysosome enriched fraction (LE fraction). At the electron microscopy (EM) level, leupeptin induced the accumulation of electron-dense vesicular structures that, in hepatocytes, were visible by 60 min after treatment (40 mg/kg). The results suggested that Leupeptin enhanced LC3b-II levels in vivo by protecting this protein from being degraded inside lysosomes, and thus the leupeptin-based assay could be potentially used for studying the dynamics of macroautophagy in mice.

Animal model

C57BL/6NCrl male mice

Formulation & Dosage

0, 9, 18 36 and 40 mg/kg; i.p. injection

References

J Antibiot (Tokyo). 1969 Nov;22(11):558-68; Cell Biol Int Rep. 1985 Sep;9(9):849-57; Plant Cell Rep. 2007 Sep;26(9):1575-84.

CAS NO:	103476-89-7
规格:	≥98%

包装	价格(元)
10mg	电议
25mg	电议
50mg	电议
100mg	电议
250mg	电议
500mg	电议
1g	电议