MD13 是一种巨噬细胞迁移抑制因子 (MIF) 导向的PROTAC,Ki值为 71 nM。MD13 可用于癌症研究。
生物活性 | MD13 is amacrophage migration inhibitory factor(MIF)-directedPROTACwith aKiof 71 nM. MD13 can be used forcancerresearch[1]. |
IC50& Target | |
体外研究 (In Vitro) | MD13 degrades 91±5% and 71±7% MIF at 2 μM and 0.2 μM, respectively. MD13 induces MIF degradation through binding to E3 ligase cereblon[1]. MD13 (0-20 μM; 72 h) inhibits cell proliferation of A549 cancer cells[1]. MD13 (1-5 μM; 48 h) arrests cell cycle at G2/M phase in A549 cells[1]. MD13 (2 μM; 6-48 h) inhibits ERK signaling[1].
Western Blot Analysis[1] Cell Line: | A549 cells | Concentration: | 0-20 μM | Incubation Time: | 12 h | Result: | Caused depletion of MIF protein. |
Western Blot Analysis[1] Cell Line: | A549 cells | Concentration: | 2 μM | Incubation Time: | 6, 24 or 48 h | Result: | Inhibited ERK phosphorylation. |
Cell Proliferation Assay[1] Cell Line: | A549 cells | Concentration: | 0-20 μM | Incubation Time: | 72 h | Result: | Inhibited the growth of A549 cells in a dose-dependent manner. The inhibitory effect reached about 50% inhibition of cell proliferation at a concentration of 20 μM. |
Cell Cycle Analysis[1] Cell Line: | A549 cells | Concentration: | 1, 2, or 5 μM | Incubation Time: | 48 h | Result: | Dose-dependently induced cell cycle arrest at the G2/M phase. |
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运输条件 | Room temperature in continental US; may vary elsewhere. |
储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |