L-Phenylalanine ((S)-2-Amino-3-phenylpropionic acid) is an essential amino acid isolated fromEscherichia coli. L-Phenylalanine is aα2δ subunit of voltage-dependent Ca⁺channelsantagonist with aK_iof 980 nM. L-phenylalanine is a competitive antagonist for the glycine- and glutamate-binding sites ofN-methyl-D-aspartate receptors (NMDARs)(K_Bof 573 μM ) andnon-NMDARs, respectively. L-Phenylalanine is widely used in the production of food flavors and pharmaceuticals^[1][2][3][4].

DAHP synthetase (DS) and chorismate mutase/prephenate dehydratase (CM/PD) are key enzymes in the L-Phenylalanine biosynthesis pathway. DS is sensitive to feedback inhibition by tyrosine, and CM/PD is subject to feedback inhibition by L-Phenylalanine^[1]. L-Phenylalanine attenuates non-NMDA receptor function in cultured neurons with an IC₅₀of 980 μM^[5].

The effects of L-Phenylalanine on NMDA-activated currents (I_NMDA) are studied in cultured hippocampal neurons from newborn rats using the patch-clamp technique. L-Phenylalanine specifically and reversibly attenuates I_NMDAin a concentration-dependent manner (IC₅₀of 1.71 mM). L-Phenylalanine inhibits specifically NMDAR current in hippocampal neurons by competing for the glycine-binding site^[3].

165.19

Solid

C₉H₁₁NO₂

63-91-2

L-苯丙氨酸；L-苯基丙氨酸

• Amino acids

Escherichia coli

Room temperature in continental US; may vary elsewhere.

H₂O : 6.67 mg/mL(40.38 mM;Need ultrasonic)

请根据半岛bd体育手机客户端 在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的半岛bd体育手机客户端 失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 9.09 mg/mL (55.03 mM); Clear solution; Need ultrasonic and warming and heat to 60℃