| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 50mg | 电议 |
Cell experiment: | Following Procarbazine or metabolite treatment, cells are diluted to 50.000/mL in 25-cm2 culture flasks (10 mL). Every 24 h, a 0.5-mL aliquot is removed, diluted 20-fold in Hematall isotonic diluent, and the cell number determined with a Coulter Model F electronic cell counter. Counts greater than 10,000/0.5 mL are corrected for coincidence. Cells are diluted in fresh culture media when cell density exceeded 1 × 106/mL. Cultures are maintained until the aggregate cell number approached 100 × 106/mL and doubling time has returned to 12 h. Cell survival is determined using Equation A, where TD(doubling time for cells of interest) is 12 h[1]. |
Animal experiment: | Mice[2]Male MutaTM Mouse animals (7-8 weeks old) are used after 2 weeks of acclimation. In the first experiment, 18 mice are injected intraperitoneally (i.p.) with 50 mg/kg Procarbazine hydrochloride in 10 mL saline/kg and eight mice are injected with 10 mL saline/kg as the vehicle control. Six treated mice are killed 7, 14, and 28 days after treatment, and four control mice are killed 7 and 28 days after the treatment. Killing is by cervical dislocation[2]. |
| 半岛bd体育手机客户端 描述 | Procarbazine Hydrochloride is an alkylating agent, with anticancer activity. Procarbazine Hydrochloride is an anticancer agent. Procarbazine is not cytotoxic to the LI 210 cells which lack cytochrome P-450 or monoamine oxidase activity[1]. Procarbazine Hydrochloride (50 mg/kg, i.p.) causes micronuclei in hematopoietic cells, but does not increase the lacZ mutant frequency (MF) in bone marrow of mice, similar to that in liver, testis, spleen, kidney, and lung. Procarbazine Hydrochloride (50 mg/kg, i.p.) has positive effect on lung, bone marrow, and spleen for carcinogenesis[2]. Procarbazine (450 mg/kg) significantly decreases testicular and epididymal weight and drastically reduces haploid cells and spermatogenic arrest in hamster[3]. References: |
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