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磷酸化丝氨酸抗体
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货号: ICP9806
品牌: ImmuneChem
参考价格: 2950
纯度含量: 大于95%
等级规格:
交货期: 现货


半岛bd体育手机客户端 介绍

磷酸化丝氨酸抗体详细介绍:

Phospho-Serine Antibody


Catalog#

Product Description

ICP9806

Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source.


Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806):Lane1-2: Kinase untreatedLane 3-6: kinase treated with phosphatase PP1GWestern blot analysis of phosphorylated proteins with UV-treated mouse cell lysates.A: ControlB-D: Treatment with increased UV doseE: Phosphatase inhibitor treatmentHigh affinity anti-phospho-serineantibody recognizes phospho-proteins (Carbonic anhydrase, phosvitin andα-casein) but not non-phospho proteins (ovalbumin, BSA,β-lactoglobulin, trypsinogen)
Species Rabbit
Formulation


250 μg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN3

Immunogen

Phospho-serine KLH and phosvitin mixture

Purification

Affinity purified with phospho-tyrosine on agarose

Specificity

The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.

Tested

Human breast cancer tissue and mouse cell

Applications

ELISA (1:250); WB (1:250); IP (10 μg/mg crude protein); IHC (1:50)

Scientific Description

Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

  1. 1.Curr. Opin. Cell. Bio.2001.13(2): 131-138.doi:10.1016/S0955-0674(00)00189-7

  2. 2.Nat. Commun. 2016.7: 11151. doi:10.1038/ncomms11151

  3. 3.Nature Communications. 2016. 7:11151: 1-15. doi:I: 10.1038/ncomms11151

  4. 4.Front Immunol.2017; 8: 352. doi: 10.3389/fimmu.2017.00352.

  5. 5.Front Immunol.2017; 8: 189. doi:10.3389/fimmu.2017.00189.

  6. 6.JAHA. 2018;7(1) doi:10.1161/JAHA.117.007185

  7. 7.AJHG. 2018.102(4): 649-657. doi:10.1016/j.ajhg.2018.02.015

  8. 8.JAHA. 2018.7(1). doi:10.1161/JAHA.117.007185

  9. 9.Cellular Physiology and Biochemisty. 2018.46(1). doi:10.1159/000488418

  10. 10.Cell Reports. 2019.28(3): 235-245. doi:10.1016/j.celrep.2019.06.045


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