Biotin-16-dCTP,orb64049
| 型号: | orb64049 | | 品牌: | biorbyt | | 参考价格: | 6539元/mL | | 纯度含量: | ≥ 95% (HPLC) | | 等级规格: | 特级 | | 包装: | 1ml | | 交货期: | 2~4周 | | 产地: | UK | | 分子式: | C32H51N8O17P3S | | 分子量: | Theoretical MW: 944.78 g/mol (free acid); Detected |
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名称 Biotin-16-dCTP(biotin)
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介绍
| Catalog Number | orb64049 |
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| Category | Tools |
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| Description | Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP. |
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| Concentration | 1.0 mM-1.1 mM |
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| Form/Appearance | filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
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| Purity | ≥ 95% (HPLC) |
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| MW | Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid) |
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| SMILES | C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2 |
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| Formula | C32H51N8O17P3S |
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| Storage | store at -20 °C |
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| Note | For research use only |
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| Application notes | Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase.
Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
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| Expiration Date | 12 months from date of receipt. |
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