质粒名称:hCas9_D10A
质粒编号:41816
质粒备注:
Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
载体名称:pcDNA3.3-TOPO
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:未知
插入基因:Cas9_D10A
基因突变:human codon-optimized, D10A nickase
插入大小:4140
启动子:CMV
克隆方法:TOPO Cloning
5'测序引物:CMV_F
质粒图谱: