5' |
|
|
G |
T 鈫掽/td> |
M |
K |
A |
C |
|
3鈥楛/td> |
|
3鈥楛/td> |
|
|
C |
A |
K |
M 鈫慄/td> |
T |
G |
|
5' |
|
FastDigest XmiI 闄愬埗鎬у唴鍒囬叾鍙瘑鍒 GT^MKAC 浣嶇偣锛屽苟浣跨敤閫氱敤 FastDigest 缂撳啿娑插湪 37鈩 涓嬬粡 5鈥?5 鍒嗛挓瀹屽叏鍒囧紑銆傚悓瑁傞叾锛欰ccI銆丗blI銆侟br>FastDigest XmiI 鏄竴绯诲垪楂樼蹇€熼檺鍒舵€у唴鍒囬叾涔嬩竴锛岃繖浜涢叾鍦ㄩ€氱敤 FastDigest 缂撳啿娑插拰 FastDigest 缁胯壊鍙嶅簲缂撳啿娑蹭腑鍧囧叿鏈 100% 娲绘€с€侟br>閫氱敤缂撳啿娑插彲鍦 5鈥?5 鍒嗛挓鍐呭揩閫熷畬鎴 DNA 鍗曢叾鍒囥€佸弻閰跺垏鎴栧閰跺垏锛屾棤闇€鏇存崲缂撳啿娑叉垨鍚庣画 DNA 绾寲姝ラ銆傚弬瑙 FastDigest 閰剁殑鍙嶅簲鏉′欢琛ㄦ牸锛屼簡瑙h閰跺拰鍏朵粬 FastDigest 闄愬埗鎬у唴鍒囬叾鐨勯叾娲绘€с€佺儹鐏椿鍜屽鑲叉椂闂淬€侱NA 淇グ閰讹紙濡 Klenow 鐗囨銆乀4 DNA 杩炴帴閰躲€佺⒈鎬х7閰搁叾鍜 T4 DNA 鑱氬悎閰讹級鍦 FastDigest 缂撳啿娑蹭腑鍧囧叿鏈 100% 娲绘€с€傚洜姝わ紝鍦ㄤ笅娓稿簲鐢ㄤ腑浣跨敤鐨勯叾鍙洿鎺ユ坊鍔犺嚦 FastDigest 鍙嶅簲娣峰悎鐗╀腑銆侟br>涓轰簡鏂逛究璧疯锛孎astDigest 缁胯壊 缂撳啿娑插寘鎷敤浜庣洿鎺ュ皢閰跺垏鍙嶅簲浜у搧涓婃牱鑷冲嚌鑳剁殑 1 绉嶅瘑搴﹁瘯鍓傚拰 2 绉嶇ず韪煋鏂欍€侟br>杈冪煭鐨勫鑲叉椂闂村拰杈冧紭鐨勯€氱敤鍨 FastDigest 缂撳啿娑茬粍鎴愭秷闄や簡鏄熸椿鎬ф晥搴斻€侟br>
鐗圭偣
?鎵€鏈 FastDigest 閰跺湪閫氱敤鍨嬬紦鍐叉恫涓殑 100% 娲绘€?br>?涓庝笅娓稿簲鐢ㄧ殑 100% 缂撳啿娑插吋瀹规€?br>? 5鈥?5 鍒嗛挓鍐呭嵆鍙畬鎴愰叾鍒嘃br>?鐩存帴涓婃牱鑷冲嚌鑳迭br>?鏃犳槦娲绘€?br>?鍙彁渚 176 绉 FastDigest 閰迭br>
搴旂敤
?鍒嗗瓙鍏嬮殕
?闄愬埗浣嶇偣浣滃浘
?鍩哄洜鍒嗗瀷
?Southern 鍗拌抗
?闄愬埗鎬х墖娈甸暱搴﹀鎬佹€ (RFLP)
? SNP 鍒嗘瀽
娉細FastDigest 缁胯壊缂撳啿娑插湪 DNA 閰跺垏鍙嶅簲鍜屼笅娓稿簲鐢ㄤ腑鍏锋湁鍜屾棤鑹 FastDigest 缂撳啿娑蹭竴鏍风殑楂樻€ц兘銆傚浜庨渶瑕侀€氳繃鑽у厜婵€鍙戣繘琛屼骇鐗╁垎鏋愮殑搴旂敤锛堜緥濡傚湪绱鍏変笅娴嬪畾娴撳害锛夛紝鍒欏缓璁娇鐢ㄦ棤鑹 FastDigest 缂撳啿娑层€侟br>鏈夊叧鐢插熀鍖栨晱鎰熸€х殑淇℃伅锛岃鍙傞槄浜у搧瑙勬牸銆侟p>
瑙勬牸
鐢插熀鍖栨晱鎰熸€?/td> |
瀵 CpG 鐢插熀鍖栨晱鎰? 瀵 Dam 鐢插熀鍖栦笉鏁忔劅, 瀵 Dcm 鐢插熀鍖栦笉鏁忔劅 |
閰迭/td> |
XmiI |
鍏煎缂撳啿娑秉/td> |
10X FastDigest 缂撳啿娑?FastDigest Green 缂撳啿娑秉/td> |
瀵圭儹澶辨椿鏁忔劅 |
鏄?/td> |
**鍙嶅簲娓╁害 |
37鈩傸/td> |
绫诲瀷 IIS RE |
鍚?/td> |
鏁伴噺 |
50 μL (50 Reactions) |
浜у搧绫诲瀷 |
闄愬埗鎬у唴鍒囬叾 |
鐮旂┒绫诲埆 |
浼犵粺鏂规硶鍏嬮殕 |
绐佸嚭绔?/td> |
5' protruding ends |
Inactivation Temperature |
65鈩傸/td> |