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搴旂敤銆怉pplication銆 :
Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
淇濊川鏈熴€恠helf life銆 :
limited shelf life, expiry date on the label
瑁呰繍銆恠hipped in銆 :
dry ice
瀛樺偍娓╁害銆恠torage temp.銆 :
-20鈩傸br/>涓汉闃叉姢瑁呭銆怭ersonal Protective Equipment銆 :
Eyeshields, Gloves
寰峰浗姘磋川鍗遍櫓鎬у垎绫汇€怶GK Germany銆 :
3