骞垮窞浼熶集绉戞妧鏈夐檺鍏徃
閾剁墝浼氬憳绗?span style="font-size:1.5em;color:#f21">6骞滁/div>
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鑱旂郴浜猴細 缃楀瓭娲?/span>
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鍦板潃锛欬span class="color3">
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鑱旂郴浜猴細 缃楀瓭娲?/span>
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鎵竴鎵紝杞绘澗鍦ㄦ墜鏈鸿闂?/div>
ROX鍙傛瘮鏌撴枡
鐢熺墿鍖栧 >> 鑽у厜鏌撴枡鍜屾番鐏墏
| 璐у彿: | 3985618 |
| 鍝佺墝: | GOYOO |
| 浜у搧鍒悕: | ROX Reference Dye |
浜у搧浠嬬粛
| Ex(nm) | 568 |
| Em(nm) | 591 |
| 鍌ㄥ瓨鏉′欢 | 閬垮厜-20鈩冮暱鏈熶繚瀛橈紝鐭湡浣跨敤鍙斁4鈩冿紝閬垮厤鍙嶅鍐昏瀺銆侟/span> |
| 杩愯緭鏉′欢 | 浣庢俯 |
| 淇濊川鏈烖/span> | 12涓湀 |
ROX Reference Dye涓€鑸敤浜嶢BI銆丼tratagene绛夊叕鍙哥殑Real Time PCR 鎵╁浠笂锛岀敤浜庤皟鏁碢CR鍔犳牱璇樊鎵€寮曡捣鐨凱CR绠′笌绠′箣闂寸殑宸紓銆備笉鍚屼华鍣ㄦ墍闇€ROX Reference Dye娴撳害涓嶅悓銆侴OYOO鎻愪緵鐨凴OX Reference Dye 鍌ㄥ瓨娑诧紝娴撳害涓?5渭mol/L锛屼娇鐢ㄧ粓娴撳害鏍规嵁涓嶅悓浠櫒涓?0nmol/L 鎴?00nmol/L锛岀敤鎴峰彲浠ユ牴鎹华鍣ㄧ殑鎺ㄨ崘娴撳害娣诲姞銆侟/span>
涓嶅悓浠櫒ROX鎺ㄨ崘浣跨敤缁堟祿搴︼細
| Instrument | ROX per 50渭L PCR Reaction | Final Concentration |
| ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One,ABI Step One Plus | 1.0渭L (0.6锝?.0渭L) | 500 nmol/L (300锝?00 nmol/L) |
| ABI 7500, 7500 Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Reseach Chromo4, Opticon (II), Corbett Rotor gene 3000 | 0.1渭L* (0.06锝?.1渭L) | 50 nmol/L (30锝?0 nmol/L) |
浠庨厤鍒禤CR鍙嶅簲鐨勬按婧舵恫涓噺鍘籖OX鏌撴枡鐨勪綋绉?/span>
*涓轰娇姣忎釜鍙嶅簲绮剧‘鍔犲叆0.1渭L锛屾帹鑽愪娇鐢ㄥ墠灏哛OX鐢ㄦ按绋€閲?0鍊嶏紝骞朵娇鐢?渭L绋€閲婂悗鐨勬憾娑层€侟/span>
鍌ㄨ繍娓╁害锛欬/strong>甯告俯杩愯緭锛?20鈩冮暱鏈熶繚瀛橈紝閬垮厤鍙嶅鍐昏瀺銆傜煭鏈熶娇鐢ㄥ彲鏀?鈩冦€侟/span>