璐у彿: | 3985612 |
鍝佺墝: | GOYOO |
绾害鍚噺: | 10,000脳 DMSO婧舵恫锛岀數娉崇骇 |
CAS NO: | 172827-25-7 |
浜у搧鍒悕: | SUPER Green II RNA gel stain |
Ex(nm) | 497 |
Em(nm) | 520 |
鍌ㄥ瓨鏉′欢 | 4鈩冨共鐕ラ伩鍏変繚瀛楛/span> |
杩愯緭鏉′欢 | 浣庢俯 |
淇濊川鏈烖/span> | 12涓湀 |
鏈搧鐢―MSO婧惰В锛屽洜DMSO鐨勭啍鐐规槸18.5鈩冿紝浣跨敤鍓嶈鏀剧疆鍒板娓╁厖鍒嗘憾瑙c€侟/strong>
SUPER Green II鏍搁吀鏌撴枡鐗圭偣
鈼 鏃犳瘨鎬э細灞炶姳绠愮被鏌撴枡锛屽鏄撶敓鐗╅檷瑙o紝鏃犺嚧鐧屾瘨鎬с€侟/span>
鈼 鐏垫晱搴﹂珮锛氳嚦灏戝彲妫€鍑?0pg ssDNA鎴朢NA锛岄珮浜嶦B鏌撹壊娉?5锝?00鍊嶃€侟/span>
鈼 淇″櫔姣旈珮锛氭牱鍝佽崸鍏変俊鍙峰己锛岃儗鏅俊鍙蜂綆銆侟/span>
鈼 鎿嶄綔绠€鍗曪細涓 EB 涓€鏍凤紝鍦ㄩ鍒惰兌鍜岀數娉宠繃绋嬩腑鏌撴枡涓嶉檷瑙o紱鑰岀數娉冲悗鏌撹壊杩囩▼涔熷彧闇€30 鍒嗛挓涓旀棤闇€鑴辫壊鎴栧啿娲楋紝鍗冲彲鐩存帴鐢ㄧ传澶栧嚌鑳堕€忓皠浠垨鍙鍏夐€忓皠浠瀵熴€侟/span>
鈼 閫傜敤鑼冨洿骞匡細鍙€夋嫨鐢垫吵鍓嶆煋鑹?鑳舵煋娉?鎴栫數娉冲悗鏌撹壊(娉℃煋娉?锛涢€傜敤浜庣惣鑴傜硸鍑濊兌鐢垫吵銆佽仛涓欑儻閰拌兒鍑濊兌鐢垫吵銆佽剦鍐茬數鍦哄嚌鑳剁數娉冲拰姣涚粏绠$數娉崇瓑锛涘彲鐢ㄤ簬 ssDNA 鎴 RNA 鏌撹壊銆侟/span>
鈼 浣跨敤鏂逛究锛氬鍒嗗瓙鐢熺墿瀛︿腑甯哥敤鐨勯叾(濡傦細Taq 閰躲€佸弽杞綍閰躲€佸唴鍒囬叾銆乀4杩炴帴閰剁瓑)娌℃湁鎶戝埗浣滅敤銆侟/span>
鈼 缁忔祹锛氫环鏍兼瘮閾舵煋渚垮疁銆侟/span>
SUPER Green II 浣跨敤鏂规硶绠€浠婞/strong>
1锛庤兌鏌撴硶(鐢ㄦ硶鍚孍B)(鎺ㄨ崘鏂规硶)
(1) 鍒惰兌鏃跺姞鍏UPER Green II 鏍搁吀鏌撴枡銆傚喎鍗磋兌鑷?0鈩冨乏鍙筹紝姣?00mL鑳朵腑鍔犲叆3锝?渭L SUPER
Green II 10,000脳 鍌ㄦ恫锛屼互姝ゆ瘮渚嬬被鎺ㄣ€侟/span>
(2) 鎸夌収甯歌鏂规硶杩涜鐢垫吵銆侟/span>
娉ㄦ剰浜嬮」锛欬/strong>
姝ゆ柟娉曟煋鑹插彲浠ュ噯纭‘瀹氭牳閰哥墖娈靛垎瀛愰噺锛屾煋鏂欑敤閲忕浉瀵硅緝灏戙€?mL鏌撴枡澶х害鍙互鍋?00鍧 100mL鐨勮兌銆侟/span>
鐢变簬SUPER Green II鐑ǔ瀹氭€ц緝宸紝涓嶈兘鍦ㄧ儹鐨勮兌婧舵恫涓洿鎺ユ坊鍔狅紝闇€瑕佺瓑寰呮憾娑插喎鍗磋嚦50鈩冨乏鍙虫墠鑳芥坊鍔犮€傛憞鏅冿紝鎸崱鎴栬€呯炕杞互淇濊瘉鏌撴枡鍏呭垎娣峰寑銆侟/span>
2锛庢场鏌撴硶
(1) 鎸夌収甯歌鏂规硶杩涜鐢垫吵銆侟/span>
(2) 鐢╬H 7.0锝?.5 鐨勭紦鍐叉恫(濡傦細TAE锛孴BE 鎴 TE)锛屾寜鐓?0000锕?鐨勬瘮渚嬬█閲奡UPER Green II 10,000脳 鍌ㄦ恫锛屾贩鍖€锛屽埗鎴?脳 鏌撹壊娑层€侟/span>
(3) 灏嗗嚌鑳跺皬蹇冨湴鏀惧叆鍚堥€傜殑瀹瑰櫒涓紝濡傝仛涓欑儻瀹瑰櫒涓€傜紦鎱㈠姞鍏ヨ冻閲忕殑1脳 鏌撹壊娑叉蹈娌″嚌鑳躲€傜敤閾濈當绛夌洊浣忓鍣ㄤ娇鏌撴枡閬垮厜銆傚娓╂尟鑽℃煋鑹?0锝?0鍒嗛挓锛屾煋鑹叉椂闂村洜鍑濊兌娴撳害鍜屽帤搴﹁€屽畾銆傝仛涓欑儻閰拌兒鍑濊兌鐩存帴鍦ㄧ幓鐠冨钩鐨夸笂鏌撹壊锛屽皢閰嶅ソ鐨?脳 鏌撹壊娑茶交杞诲湴鍊掑湪鑳舵澘涓婏紝璁╁叾鍧囧寑鍦拌鐩栨暣涓兌鏉匡紝骞舵煋鑹?0鍒嗛挓銆傜幓鐠冨钩鐨垮繀椤婚鍏堢粡杩囩鐑峰寲婧舵恫澶勭悊(閬垮厤鏌撴枡鍚搁檮鍦ㄧ幓鐠冭〃闈笂)銆侟/span>
娉細鐢ㄦ场鏌撴硶鏌撹壊鏃讹紝鍙互绮剧‘纭畾鏍搁吀鐗囨鍒嗗瓙閲忋€備絾鏌撴枡鐢ㄩ噺杈冨銆侟/span>