Ex(nm)	304
Em(nm)	608
鍌ㄥ瓨鏉′欢	4鈩冨共鐕ラ伩鍏変繚瀛楛/span>
杩愯緭鏉′欢	浣庢俯
淇濊川鏈烖/span>	12涓湀

CelRed鏍搁吀鏌撴枡鐗圭偣

鈼 鏃犳瘨鎬э細CelRed 鐙壒鐨勬补鎬у拰澶у垎瀛愰噺鐗圭偣浣垮叾涓嶈兘绌块€忕粏鑳炶啘杩涘叆缁嗚優鍐咃紝鑹惧鏂皬璇曢獙缁撴灉涔熻〃鏄庯紝璇ユ煋鏂欑殑璇卞彉鎬ц繙杩滃皬浜嶦B銆侟/span>

鈼 鐏垫晱搴﹂珮锛氶€傜敤浜庡悇绉嶅ぇ灏忕墖娈电殑鐢垫吵鏌撹壊锛屽鏍搁吀杩佺Щ鐨勫奖鍝嶅皬浜嶴YBR Green I銆侟/span>

鈼 绋冲畾鎬ч珮锛 閫傜敤浜庝娇鐢ㄥ井娉㈡垨鍏跺畠鍔犵儹鏂规硶鍒跺鐞艰剛绯栧嚌鑳讹紱瀹ゆ俯涓嬪湪閰告垨纰辩紦鍐叉恫涓瀬鍏剁ǔ瀹氾紝鑰愬厜鎬у己銆侟/span>

鈼 淇″櫔姣旈珮锛氭牱鍝佽崸鍏変俊鍙峰己锛岃儗鏅俊鍙蜂綆銆侟/span>

鈼 鎿嶄綔绠€鍗曪細涓 EB 涓€鏍凤紝鍦ㄩ鍒惰兌鍜岀數娉宠繃绋嬩腑鏌撴枡涓嶉檷瑙ｏ紱鑰岀數娉冲悗鏌撹壊杩囩▼涔熷彧闇€30 鍒嗛挓涓旀棤闇€鑴辫壊鎴栧啿娲 锛屽嵆鍙洿鎺ョ敤绱鍑濊兌閫忓皠浠瀵熴€侟/span>

鈼 閫傜敤鑼冨洿骞匡細鍙€夋嫨鐢垫吵鍓嶆煋鑹?鑳舵煋娉?鎴栫數娉冲悗鏌撹壊(娉℃煋娉?锛涢€傜敤浜庣惣鑴傜硸鍑濊兌鎴栬仛涓欑儻閰拌兒鍑濊兌鐢垫吵锛涘彲鐢ㄤ簬 dsDNA銆乻sDNA 鎴 RNA 鏌撹壊銆侟/span>

鈼 涓嶦B鏈夌浉鍚岀殑鍏夎氨鐗规€э紝鏃犻渶鏀瑰彉婊ゅ厜鐗囧強瑙傚療瑁呯疆锛氭爣鍑嗙殑 EB 婊ゅ厜鐗囨垨 SYBR 婊ゅ厜鐗囬兘閫傜敤锛屼娇鐢ㄤ笌瑙傚療EB鐩稿悓鐨勬櫘閫氱传澶栧嚌鑳堕€忓皠浠瀵熷嵆鍙紝鍦 300nm 绱鍏夐檮杩戝彲寰楀埌鏈€浣虫縺鍙戙€備絾鏄疌elRed涓嶈兘琚?88 nm姘╃瀛愭縺鍏夊櫒鎴栫浉浼兼尝闀跨殑鍙鍏夊畬鍏ㄦ縺鍙戯紝鍥犳涓嶆帹鑽愪娇鐢ㄦ绫绘縺鍙戣缃殑鎴愬儚绯荤粺銆傚浜庢绫昏缃紝鎴戜滑鎺ㄨ崘鎮ㄤ娇鐢–elGreen(Cat# 011鎴?12)锛屽畠鍜孲YBR Green I鐨勫厜璋辩浉浼硷紝鐏垫晱搴︾浉褰擄紝浣嗘洿鍔犵ǔ瀹氥€侟/span>

CelRed浣跨敤鏂规硶绠€浠婞/strong>

1銆佽兌鏌撴硶(鐢ㄦ硶鍚孍B)(鎺ㄨ崘鏂规硶)

(1) 鍒惰兌鏃跺姞鍏elRed 鏍搁吀鏌撴枡(渚嬪锛氭瘡50mL 鐞艰剛绯栨憾娑蹭腑鍔犲叆5渭L CelRed 10,000脳 鍌ㄦ恫锛孅/span>

浠ユ姣斾緥绫绘帹)銆侟/span>

(2) 鎸夌収甯歌鏂规硶杩涜鐢垫吵銆侟/span>

娉ㄦ剰浜嬮」锛欬/span>

姝ゆ柟娉曟煋鑹叉煋鏂欑敤閲忕浉瀵硅緝灏戙€?00 渭L鏌撴枡澶х害鍙互鍋?00鍧 50mL鐨勮兌銆侟/span>

鐢变簬CelRed鍏锋湁鑹ソ鐨勭儹绋冲畾鎬э紝鍙互鍦ㄧ儹鐨勭惣鑴傜硸婧舵恫涓洿鎺ユ坊鍔狅紝鑰屼笉闇€瑕佺瓑寰呮憾娑插喎鍗淬€傛憞鏅冿紝鎸崱鎴栬€呯炕杞互淇濊瘉鏌撴枡鍏呭垎娣峰寑銆備篃鍙互閫夋嫨灏咰elRed鍌ㄦ恫鍔犲埌鐞艰剛绯栫矇鏈拰鐢垫吵缂撳啿娑蹭腑锛岀劧鍚庣敤寰尝鐐夋垨鍏朵粬甯哥敤鏂瑰紡鍔犵儹浠ュ埗澶囩惣鑴傜硸鍑濊兌銆侰elRed鍏煎鎵€鏈夊父鐢ㄧ殑鐢垫吵缂撳啿婧舵恫銆侟/span>

濡傛灉鎬绘槸鐪嬪埌鏉″甫寮ユ暎鎴栧垎绂讳笉鐞嗘兂锛屽缓璁娇鐢ㄦ场鏌撴硶鏌撹壊浠ョ‘璁ら棶棰樻槸鍚︿笌鏌撴枡鏈夊叧銆傚鏋滄煋鑹插悗闂渚濇棫瀛樺湪锛屽垯璇存槑闂涓庢煋鏂欐棤鍏筹紝璇峰皾璇曪細闄嶄綆鐞艰剛绯栨祿搴︼紱閫夌敤鏇撮暱鐨勫嚌鑳讹紱寤堕暱鍑濊兌鏃堕棿浠ヤ繚璇佽竟缂樻竻鏅帮紱鏀硅繘涓婃牱鎶€宸ф垨閫夋嫨娉℃煋娉曟煋鑹层€侟/span>

姝ゆ柟娉曚笉閫傚悎棰勫埗鑱氫笝鐑叞鑳哄嚌鑳讹紝瀵逛簬鑱氫笝鐑叞鑳哄嚌鑳惰浣跨敤娉℃煋娉曘€侟/span>

2銆佹场鏌撴硶

(1) 鎸夌収甯歌鏂规硶杩涜鐢垫吵銆侟/span>

(2) 鐢℉2O灏咰elRed 10,000脳 鍌ㄦ恫绋€閲婄害3,300鍊嶅埌0.1M NaCl涓紝鍒舵垚3脳 鏌撹壊娑层€?渚嬪灏?5渭L CelRed 10,000脳 鍌ㄦ恫鍜?mL 1M NaCl鍔犲埌45mL H2O涓?銆侟/span>

(3) 灏嗗嚌鑳跺皬蹇冨湴鏀惧叆鍚堥€傜殑瀹瑰櫒涓紝濡傝仛涓欑儻瀹瑰櫒涓€傜紦鎱㈠姞鍏ヨ冻閲忕殑3脳 鏌撹壊娑叉蹈娌″嚌鑳躲€傚娓╂尟鑽℃煋鑹?0min宸﹀彸锛屾渶浣虫煋鑹叉椂闂存牴鎹嚌鑳跺帤搴︿互鍙婄惣鑴傜硸娴撳害涓嶅悓鑰岀暐鏈変笉鍚屻€傚浜庡惈3.5锝?0%涓欑儻閰拌兒鐨勫嚌鑳讹紝鏌撹壊鏃堕棿閫氬父浠嬩簬30min鍒?h锛屽苟闅忎笝鐑叞鑳哄惈閲忓鍔犺€屽欢闀裤€侟/span>

娉ㄦ剰浜嬮」锛欬/span>

鐢ㄦ场鏌撴硶鏌撹壊鏃讹紝鏌撴枡鐢ㄩ噺杈冨銆傚崟娆′娇鐢ㄧ殑鏌撹壊娑插彲閲嶅浣跨敤3娆″乏鍙炽€侟/span>

3脳 CelRed鏌撹壊娑插彲浠ュぇ閲忓埗澶囷紝鍦ㄥ娓╀笅閬垮厜淇濆瓨鐩磋嚦鐢ㄥ畬銆侟/span>

鍑犵鏍搁吀鏌撴枡姣旇緝

鍚嶇О	鐏垫晱搴?/span>	绋冲畾鎬?/span>	瀵笵NA杩佺Щ鐨勫奖鍝岦/span>	瀹夊叏鎬?/span>	閫傜敤鎬?/span>
CelRed	楂楛/span>	楂楛/span>	鏉″甫涓嶅集鏇诧紝DNA鐗囨涓嶈縼绉狐/span>	鈶 鏄竴绉嶇嫭鐗圭殑娌规€уぇ鍒嗗瓙锛屼笉鏄撴尌鍙戝崌鍗庯紝涓嶆槗鍚稿叆浜轰綋锛屼笉鑳界┛閫忕粏鑳炶啘杩涘叆娲讳綋缁嗚優鍐咃紝瀹夊叏鏃犳瘨銆傗憽 鑹惧鏂皬娴嬭瘯缁撴灉琛ㄦ槑锛孋elRed鍦ㄥ嚌鑳舵煋鑹叉祿搴︿笅瀹屽叏娌℃湁璇卞彉鎬э紝鍥犳瀹屽叏娌℃湁鑷寸檶姣掓€с€侟/span>	閫氱敤澶у皬鐗囨鐢垫吵鏌撹壊锛屼笌EB鏈夌浉鍚岀殑鍏夎氨鐗规€э紝鏃犻渶鏀瑰彉婊ゅ厜鐗囧強瑙傚療瑁呯疆銆傛爣鍑嗙殑EB婊ゅ厜鐗囨垨SYBR婊ゅ厜鐗囬兘閫傜敤锛屼娇鐢ㄦ櫘閫氱传澶栧嚌鑳堕€忓皠浠瀵熷嵆鍙紝鍦?00nm绱鍏夐檮杩戝彲寰楀埌鏈€浣虫縺鍙戙€侟/span>
CelGreen	楂楛/span>	楂楛/span>	鏉″甫涓嶅集鏇诧紝DNA鐗囨涓嶈縼绉狐/span>	鈶 鏄竴绉嶇嫭鐗圭殑娌规€уぇ鍒嗗瓙锛屼笉鏄撴尌鍙戝崌鍗庯紝涓嶆槗鍚稿叆浜轰綋锛屼笉鑳界┛閫忕粏鑳炶啘杩涘叆娲讳綋缁嗚優鍐咃紝瀹夊叏鏃犳瘨銆傗憽 鑹惧鏂皬娴嬭瘯缁撴灉琛ㄦ槑锛孋elGreeri鍦ㄥ嚌鑳舵煋鑹叉祿铏斾笅瀹屽叏娌℃湁璇卞彉鎬э紝鍥犳瀹屽叏娌℃湁鑷寸檶姣掓€с€侟/span>	閫氱敤澶у皬鐗囨鐢垫吵鏌撹壊锛岄€傜敤浜庝娇鐢?54nm婵€鍙戠殑绱鍑濊兌閫忓皠浠垨鍙鍏夊嚌鑳堕€忓皠浠瀵熴€侟/span>
SYBR Green I	楂楛/span>	浣嶞/span>	鏌撴枡娴撳害杈冮珮鏃讹紝鏉″甫瀹规槗寮洸锛孌NA鐗囨杩佺Щ鐨勭幇璞℃槑鏄近/span>	灞炶姳绠愭煋鏂欙紝鑳介€忚繃缁嗚優鑶滆繘鍏ユ椿浣撶粏鑳炲唴锛屼絾瀹规槗鐢熺墿闄嶈В锛屼笉浼氬湪浣撳唴娈嬬暀锛屽畨鍏ㄦ棤姣掋€侟/span>	lOObp浠ヤ笂鐢垫吵鏌撹壊锛岄€傜敤浜庝娇鐢?54nm婵€鍙戠殑绱鍑濊兌閫忓皠浠垨鍙鍏夊嚌鑳堕€忓皠浠瀵熴€侟/span>
EB	浣嶞/span>	楂楛/span>	鏉″甫涓嶅集鏇诧紝DNA鐗囨涓嶈縼绉狐/span>	鈶 鍒嗗瓙閲忓皬锛屾槗鎸ュ彂鍗囧崕锛屾槗鍚稿叆浜轰綋锛屼笉鏄撶敓鐗╅檷瑙ｏ紝鍦ㄤ綋鍐呴暱鏈熸畫鐣欍€傗憽 鑹惧鏂皬娴嬭瘯缁撴灉琛ㄦ槑锛孍B瀹规槗寮曡捣鏈夋満浣撶獊鍙橈紝鏄竴绉嶅己璇卞彉鍓傦紝鍏锋湁楂樿嚧鐧屾€с€侟/span>	lOObp浠ヤ笂鐢垫吵鏌撹壊锛岃儗鏅崸鍏変俊鍙烽珮锛涗娇鐢ㄦ櫘閫氱传澶栧嚌鑳堕€忓皠浠瀵熴€侟/span>
Goldview	浣嶞/span>	楂楛/span>	鏉″甫涓嶅集鏇诧紝DNA鐗囨涓嶈縼绉狐/span>	鈶 涓昏鎴愬垎涓哄悥鍟舵锛屾槸涓€绉嶇叅鐒︽补鎻愬彇鐗╋紝鍏锋湁楂樻瘨锛岃嚧鐧岋紝楂樿鍙樻€с€傗憽 鏄撴尌鍙戝崌鍗庯紝鏄撳惛鍏ヤ汉浣擄紝鑳界┛閫忕粏鑳炶啘杩涘叆娲讳綋缁嗚優鍐咃紝涓嶆槗鐢熺墿闄嶈В锛屽湪浣撳唴闀挎湡娈嬬暀銆侟/span>	lOObp浠ヤ笂鐢垫吵鏌撹壊锛岃儗鏅崸鍏変俊鍙烽珮锛涢€傜敤浜庝娇鐢?54nm婵€鍙戠殑绱鍑濊兌閫忓皠浠垨鍙鍏夊嚌鑳堕€忓皠浠瀵熴€侟/span>

鐗瑰埆鎻愰啋锛欬/strong>

濡傛灉鎮ㄤ娇鐢ㄧ殑鏄传澶栨垚鍍忎华锛岃閫夋嫨CelRed锛涘鏋滄偍浣跨敤婵€鍏夋垚鍍忎华鎴栧笇鏈涘湪鍙鍏変笅瑙傛祴锛岃閫夋嫨 CelGreen銆侟/span>

鍦ㄦ瀬灏戞暟鎯呭喌涓嬶紝璐ㄧ矑缁忔煇浜涢叾鍒囧悗鐨凞NA鏍峰搧浼氬嚭鐜版嫋灏惧拰鍒嗚鲸鐜囬檷浣庯紝姝ゆ椂寤鸿鍚屾椂灏濊瘯涓ょ鏌撹壊鏂规硶浠ュ喅瀹氬摢绉嶆柟娉曟洿鍔犲悎閫傘€侟/span>