鍒嗗瓙寮廃/span>	C32H37N4S
鍒嗗瓙閲廃/span>	509.73
Ex(nm)	497
Em(nm)	525
鍌ㄥ瓨鏉′欢	4鈩冨共鐕ラ伩鍏変繚瀛橈紙鐢垫吵绾э級 -20鈩冨共鐕ラ伩鍏変繚瀛橈紙PCR绾э級
杩愯緭鏉′欢	浣庢俯
淇濊川鏈烖/span>	12涓湀

鏈搧鐢―MSO婧惰В锛屽洜DMSO鐨勭啍鐐规槸18.5鈩冿紝浣跨敤鍓嶈鏀剧疆鍒板娓╁厖鍒嗘憾瑙ｃ€侟/strong>

SUPER Green I鏍搁吀鏌撴枡鐗圭偣

鈼 鏃犳瘨鎬э細灞炶姳绠愮被鏌撴枡锛屽鏄撶敓鐗╅檷瑙ｏ紝鏃犺嚧鐧屾瘨鎬с€侟/span>

鈼 鐏垫晱搴﹂珮锛氳嚦灏戝彲妫€鍑?0pg DNA锛岄珮浜嶦B鏌撹壊娉?5锝?00鍊嶃€侟/span>

鈼 淇″櫔姣旈珮锛氭牱鍝佽崸鍏変俊鍙峰己锛岃儗鏅俊鍙蜂綆銆侟/span>

鈼 鎿嶄綔绠€鍗曪細鏃犻』鑴辫壊鎴栧啿娲楋紝鍗冲彲鐩存帴鐢ㄧ传澶栧嚌鑳堕€忓皠浠垨鍙鍏夐€忓皠浠瀵熴€侟/span>

鈼 閫傜敤鑼冨洿骞匡細鍙€傜敤浜庡绉嶅嚌鑳剁數娉虫柟娉曪細鐞艰剛绯栧嚌鑳躲€佽仛涓欑儻閰拌兒鍑濊兌鐢垫吵銆佽剦鍐茬數鍦哄嚌鑳剁數娉冲拰姣涚粏绠＄數娉崇瓑銆侟/span>

鈼 浣跨敤鏂逛究锛氬鍒嗗瓙鐢熺墿瀛︿腑甯哥敤鐨勯叾(濡傦細Taq 閰躲€佸弽杞綍閰躲€佸唴鍒囬叾銆乀4杩炴帴閰剁瓑)娌℃湁鎶戝埗浣滅敤銆侟/span>

鈼 缁忔祹锛氫环鏍兼瘮閾舵煋渚垮疁銆侟/span>

SUPER Green I 浣跨敤鏂规硶绠€浠婞/strong>

1銆佽兌鏌撴硶(鐢ㄦ硶鍚孍B)

(1) 鍒惰兌鏃跺姞鍏UPER Green I 鏍搁吀鏌撴枡銆傚喎鍗磋兌鑷?0鈩冨乏鍙筹紝姣?00mL鑳朵腑鍔犲叆3锝?渭L

SUPER Green I 鏍搁吀鏌撴枡銆侟/span>

(2) 鎸夌収甯歌鏂规硶杩涜鐢垫吵鍗冲彲銆侟/span>

娉細姝ゆ柟娉曟煋鑹插彲浠ュ噯纭‘瀹氭牳閰哥墖娈靛垎瀛愰噺锛屾煋鏂欑敤閲忕浉瀵硅緝灏戙€?mL鏌撴枡澶х害鍙互鍋?00鍧 100mL鐨勮兌銆侟/span>

2銆佺偣鏌撴硶

(1) 璇ユ柟娉曢€備簬鐞艰剛绯栧嚌鑳剁數娉冲拰PAGE鍑濊兌鐢垫吵銆侟/span>

(2) 宸ヤ綔娑茬殑閰嶅埗锛氱敤鐢垫吵缂撳啿娑插皢10000脳鐨凷UPER Green I 绋€閲?00鍊嶏紝鍗充负SUPER Green I 宸ヤ綔娑层€係UPER Green I 宸ヤ綔娑插彲浠ョ疆2锝?鈩冧繚瀛樹竴涓湀浠ヤ笂銆侟/span>

(3) 鍒惰兌锛氭寜甯歌鏂规硶鍒惰兌锛屼笉鍚换浣曟煋鏂欍€侟/span>

(4) 鏍峰搧鏌撹壊锛氬悜鍒嗘瀽鏍峰搧涓姞鍏UPER Green I 宸ヤ綔娑插拰杞芥牱缂撳啿娑诧紝瀹ゆ俯鏀剧疆10鍒嗛挓锛屼娇SUPER Green I 涓庢牱鍝佷腑DNA鍏呭垎缁撳悎銆係UPER Green I 宸ヤ綔娑插姞鍏ラ噺涓烘€讳笂鏍烽噺鐨?/5锝?/10銆侟/span>

(5) DNA Marker鏌撹壊锛氬皢5渭L DNA Marker銆?渭L DNA Marker绋€閲婃恫鍜?渭LSUPER Green I 宸ヤ綔娑叉贩鍖€锛屽娓╂斁缃?鍒嗛挓锛屼娇SUPER Green I 涓嶥NA鍏呭垎缁撳悎銆侟/span>

(6) 涓婃牱銆佺數娉筹細鎸夊父瑙勬搷浣溿€侟/span>

娉細鐢ㄧ偣鏌撴硶鏌撹壊鏃讹紝鐏垫晱搴︽渶楂橈紝鏌撴枡鐢ㄩ噺鏈€灏戙€備絾澶х墖娈电◢鏈夋粸鍚庣幇璞★紝濡傛灉闇€瑕佹洿鍑嗙‘纭畾鍒嗗瓙閲?涓嶮arker瀵规瘮)锛屽缓璁娇鐢ㄨ兌鏌撴硶銆侟/span>

3銆佹场鏌撴硶

(1) 鎸夌収甯歌鏂规硶杩涜鐢垫吵銆侟/span>

(2) 鐢╬H 7.0锝?.5 鐨勭紦鍐叉恫(濡傦細TAE锛孴BE 鎴 TE)锛屾寜鐓?0000锕?鐨勬瘮渚嬬█閲奡UPER Green I 娴撶缉娑诧紝娣峰寑锛屽埗鎴愭煋鑹叉憾娑层€侟/span>

(3) 灏嗘煋鑹叉憾娑插€掑叆鍚堥€傜殑鑱氫笝鐑鍣ㄤ腑锛屾斁鍏ュ嚌鑳讹紝鐢ㄩ摑绠旂瓑鐩栦綇瀹瑰櫒浣挎煋鏂欓伩鍏夈€傚娓╂尟鑽℃煋鑹?0锝?0鍒嗛挓锛屾煋鑹叉椂闂村洜鍑濊兌娴撳害鍜屽帤搴﹁€屽畾銆傝仛涓欑儻閰拌兒鍑濊兌鐩存帴鍦ㄧ幓鐠冨钩鐨夸笂鏌撹壊锛屽皢閰嶅ソ鐨勭█閲婃憾娑茶交杞诲湴鍊掑湪鑳舵澘涓婏紝璁╃█閲婃恫鍧囧寑鍦拌鐩栨暣涓兌鏉匡紝骞舵煋鑹?0鍒嗛挓銆傜幓鐠冨钩鐨垮繀椤婚鍏堢粡杩囩鐑峰寲婧舵恫澶勭悊(閬垮厤鏌撴枡鍚搁檮鍦ㄧ幓鐠冭〃闈笂)銆侟/span>

娉細鐢ㄦ场鏌撴硶鏌撹壊鏃讹紝鍙互绮剧‘纭畾鏍搁吀鐗囨鍒嗗瓙閲忋€備絾鏌撴枡鐢ㄩ噺鏄笁绉嶆柟娉曚腑鏈€澶氱殑銆侟/span>

4銆 鐐规煋锛嬭兌鏌撴硶

姝ゆ硶缁撳悎鏂规硶1鍜屾柟娉?锛岀伒鏁忓害鏈€楂橈紝瀵逛簬浣庢祿搴︽牱鏈瘮EB妫€娴嬫洿鐏垫晱銆侟/span>

鍑犵鏌撹壊鏂规硶鐗圭偣姣旇緝

鐗圭偣	鐏垫晱搴?/span>	鏌撴枡鐢ㄩ噺	纭畾鐗囨鍒嗗瓙閲忕簿纭害
鏌撹壊鏂规硶
鑳舵煋娉?鎺ㄨ崘)	杈冮珮	杈冨皯	杈冮珮
鐐规煋娉旤/span>	寰堥珮	鏈€灏慄/span>	澶х墖娈电◢鏈夋粸鍚嶞/span>
娉℃煋娉旤/span>	杈冮珮	鏈€澶欬/span>	鏈€楂楛/span>
鐐规煋+鑳舵煋娉旤/span>	鏈€楂楛/span>	杈冨	澶х墖娈电◢鏈夋粸鍚嶞/span>

SUPER Green I 浣跨敤娉ㄦ剰浜嬮」

(1) 鍦⊿UPER Green I鐐规煋娉曚腑锛岀數娉虫椂闂翠笉瑕佽秴杩?灏忔椂锛屽惁鍒橲UPER Green I浼氫粠DNA涓婂垎绂诲嚭鏉ワ紝浼氫骇鐢熷讥鏁ｇ姸鏉″甫銆侟/span>

(2) 鐢ㄧ偣鏌撴柟娉曟煋鑹叉椂锛屾潯甯︾◢鏈夋粸鍚庣幇璞★紝濡傛灉闇€瑕佺‘瀹氱墖娈电簿纭垎瀛愰噺(涓嶮arker瀵规瘮)锛屽缓璁娇鐢ㄨ兌鏌撴硶(鏂规硶1)銆侟/span>

(3) 鍦ㄥ父瑙勭敤閰掔簿娌夋穩鏍搁吀鐨勮繃绋嬩腑锛孲UPER Green I鍙互鍏ㄩ儴浠庡弻閾炬牳閰镐笂鍘绘帀銆侟/span>

(4) 濡傛灉鎯冲鐢 SUPER Green I 鏌撹繃鐨勮兌杩涜 Southern blots锛屽缓璁湪棰勬潅鍖栧拰鏉傚寲婧舵恫涓姞鍏 0.1%锝?.3% 鐨凷DS銆侟/span>

(5) 鍦ㄧ传澶栫収灏勯€忚涓嬶紝涓庡弻閾 DNA 鎺ュ悎鐨 SUPER Green I鍛堢幇缁胯壊鑽у厜銆傚鏋滆兌涓惈鏈夊崟閾 DNA 鍒欓鑹蹭负姗橀粍鑰屼笉鏄豢鑹层€侟/span>

(6) SUPER Green I瀵圭幓鐠冨拰闈炶仛涓欑儻鏉愭枡鍏锋湁涓€瀹氫翰鍚堝姏銆傚缓璁湪绋€閲娿€佽串瀛樸€佹煋鑹茬瓑浣跨敤杩囩▼涓敤鑱氫笝鐑被瀹瑰櫒銆侟/span>