璁よ瘉锛欬span class="color3">宸ュ晢淇℃伅宸叉牳瀹濣/span>
鑱旂郴浜猴細 缃楀瓭娲?/span>
鐢佃瘽锛欬span class="color3">15323361772
鍦板潃锛欬span class="color3">
鍝佺墝: GOYOO
绾害鍚噺: 鈮?5% / 5mM 鏃犳按DMSO婧舵恫
鍖呰: 100渭g锛?mg锛?0脳100渭g锛?0渭L
绱㈠彇璧勬枡鍙婃姤浠饵/span>
| 璐у彿: | 3985693 |
| 鍝佺墝: | GOYOO |
| 绾害鍚噺: | 鈮?5% / 5mM 鏃犳按DMSO婧舵恫 |
| CAS NO: | 273221-67-3 |
| 浜у搧鍒悕: | Fluo-4, AM 1-[2-Amino-5-(2,7-difluoro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, pentaacetoxymethyl ester |
| 鍒嗗瓙寮廃/span> | C51H50F2N2O23 |
| 鍒嗗瓙閲廃/span> | 1096.94 |
| Ex(nm) | 494 |
| Em(nm) | 516 |
| 鍌ㄥ瓨鏉′欢 | -20鈩冨共鐕ラ伩鍏変繚瀛楛/span> |
| 杩愯緭鏉′欢 | 甯告俯 |
| 淇濊川鏈烖/span> | 12涓湀 |
Fluo-4鏄竴绉嶅皢Fluo-3缁撴瀯涓殑Cl鏇挎崲鎴怓鐨勯挋鑽у厜鎺㈤拡銆傜敱浜庡皢Cl鏇挎崲鎴愪簡鐢靛瓙鍚稿紩鍔涙洿寮虹殑F锛屽畠鐨勬渶澶ф縺鍙戞尝闀夸細鍚戠煭娉㈤暱澶勫亸绂?0 nm宸﹀彸銆傝繖涓尝闀挎洿鎺ヨ繎浜庢癌婵€鍏夊櫒鐨勬尝闀匡紝鎵€浠ョ敤姘╂縺鍏夊櫒婵€鍙戞椂锛孎luo-4鐨勮崸鍏夊己搴︽瘮Fluo-3寮轰竴鍊嶃€侟/span>
Fluo-4, AM绌块€忕粏鑳炶啘杩涘叆缁嗚優鍚庤缁嗚優鍐呯殑閰叾鍓垏褰㈡垚Fluo-4锛屼粠鑰岃婊炵暀鍦ㄧ粏鑳炲唴锛孎luo-4鑻ヤ互娓哥閰嶄綋褰㈠紡瀛樺湪鏃跺嚑涔庢槸闈炶崸鍏夋€х殑锛屼絾鏄綋瀹冧笌缁嗚優鍐呴挋绂诲瓙缁撳悎鍚庡彲浠ヤ骇鐢熻緝寮虹殑鑽у厜锛屾渶澶ф縺鍙戞尝闀夸负494nm锛屾渶澶у彂灏勬尝闀夸负516nm銆傚彲浠ヤ娇鐢ㄦ縺鍏夊叡鑱氱劍鏄惧井闀滄垨娴佸紡缁嗚優浠娴嬬粏鑳炲唴閽欑瀛愭祿搴︾殑鍙樺寲銆侟/span>
鎿嶄綔璇存槑(for Human T cells)*
(1) 璇曞墏
2 mM鐨凢luo-4, AM/DMSO(灏?mg Fluo-4, AM婧朵簬442渭L DMSO)
Pluronic F127
Hanks?balanced salt solution(HBSS)
HEPES buffer saline(10mM HEPES锛?mM Na2HPO4锛?37mM NaCl锛?mM KCl锛?mM CaCl2锛?.5mM MgCl2锛?mM glucose锛?.1% BSA锛宲H 7.4)
(2) 鎿嶄綔
鈶? 鍚慒luo-4, AM/DMSO婧舵恫涓姞鍏?6.5mg Pluronic F127锛孭luronic F127鍙互 闃叉Fluo-4, AM鍦℉BSS涓仛鍚堝苟鑳藉府鍔╁叾杩涘叆缁嗚優銆侟/span>
鈶? 鐢℉BSS绋€閲奆luo-4, AM婧舵恫锛屽埗澶?渭M鐨凢luo-4, AM宸ヤ綔娑层€侟/span>
鈶? 灏咶luo-4, AM宸ヤ綔娑插姞鍏ョ粏鑳烇紝鍦?7鈩冨煿鍏?0鍒嗛挓銆侟/span>
鈶? 鍔犲叆5鍊嶄綋绉殑鍚湁1%鑳庣墰琛€娓呯殑HBSS锛屽啀缁х画鍩瑰吇40鍒嗛挓銆侟/span>
鈶? 鐢℉EPES buffer saline娲楁钉缁嗚優3娆★紝鐒跺悗鐢℉EPES buffer saline浣跨粏鑳為噸鎮诞锛屽埗鎴?脳105 cells/mL鐨勬憾娑层€侟/span>
鈶? 37鈩冧笅鍩瑰吇10鍒嗛挓锛岀劧鍚庣敤璇ョ粏鑳炶繘琛岃崸鍏夐挋绂诲瓙妫€娴嬨€傛縺鍙戞尝闀?94nm锛屽彂灏勬尝闀?16nm銆侟/span>
*鏍囪鐨勬潯浠跺洜缁嗚優绉嶇被鑰屽紓锛屽湪姣忔瀹為獙鍓嶏紝璇峰厛纭畾鏈€浣虫潯浠躲€備互涓婃柟娉曚粎渚涘弬鑰冦€侟/span>
鍌ㄥ瓨鏉′欢锛?20鈩冨共鐕ラ伩鍏変繚瀛橈紝鏈夋晥鏈熶竴骞淬€侟/span>