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Insulin-Transferrin-Sodium Selenite Supplement
| 半岛bd体育手机客户端 编号： | 4157524 |
| 规格： | suitable for cell culture, lyophilized, pkg of 50 mg (for 5 l medium) |
| 包装规格： | 50 MG |
| 半岛bd体育手机客户端 类别： | 进口试剂 |
| 品牌： | Roche |
| 优惠价： | 立即咨询 |
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性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | human serum (transferrin) synthetic (sodium selenite) |
| recombinant【重组】 | expressed in human cells |
| sterility【无菌性】 | non-sterile; 0.2 μm filtered |
| Assay【测定】 | >98% (Transferrin, SDS-PAGE) >99% (Insulin, HPLC) >99% (Sodium selenite) |
| form【形式】 | lyophilized |
| packaging【包装】 | pkg of 50 mg (for 5 l medium) |
| manufacturer/tradename | Roche |
| technique(s) | cell culture | mammalian: suitable |
| solubility【溶解性】 | water: miscible |
| UniProt accession no.【UniProt登记号】 | P01317 P02786 |
| shipped in【运输】 | wet ice |
| storage temp.【储存温度】 | 2-8℃ |
| Gene Information | bovine ... INS(280829) human ... TFRC(7037) |
基本信息
| General description【一般描述】 | The insulin-transferrin-sodium selenite (ITS) supplement comprises three of the most crucial growth factors for many cell types. Insulin is a component of serum-free media formulations for all primary cells and cell lines. It stimulates cell growth, increases fatty acid and glycogen synthesis in a serum-free medium. Transferrin is an essential growth factor for many cell types. Selenium is very often necessary for optimal cell growth. ITS permits an appreciable reduction in serum requirements for cellular growth. ITS is also an antioxidant that supports the growth of in vivo derived (IVD) embryos. Insulin promotes embryonic growth and metabolism. The iron transport in the embryo is mediated by transferrin. Sodium selenite protects from oxidative damage and inhibits lipid peroxidation. ITS is useful in human chondrocytes monolayer culture. The Insulin-Transferrin-Sodium Selenite Supplement contains the most essential growth-promoting components of serum-free culture media in an optimized ratio. Source: Insulin- bovine pancreas; transferrin- human serum; Sodium selenite-synthetic |
| Application【应用】 | The Insulin-Transferrin-Sodium Selenite Supplement is used in the cell culture media as a basal growth-factor supplement to which other cell type-specific nutrients and growth factors are added. |
| Preparation Note【制备说明】 | Working concentration: Insulin, 5 μg/ml, transferrin, 5 μg/ml, sodium selenite, 5 ng/ml (3.0 x 10-8 M) Storage conditions (working solution): -15 to -25 ℃. Prepare appropriate aliquots and avoid repeated freezing and thawing. |
| Reconstitution【重悬】 | The insulin-transferrin-sodium selenite supplement should be reconstituted in 5 ml or 25 ml sterile double-dist. water (1000 x concentrated stock solution). Further dilution with culture medium. |
| Other Notes【其他说明】 | For life science research only. Not for use in diagnostic procedures. |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 1 |
