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Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG)
| 半岛bd体育手机客户端 编号： | 4154990 |
| 规格： | Chemicon?, from rabbit |
| 包装规格： | 100 μG |
| 半岛bd体育手机客户端 类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| eCl@ss |
32160702
|
| NACRES |
NA.43
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| Specificity【特异性】 |
T7 (MASMTGGQQMG). Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
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| Immunogen【免疫原】 |
Epitope: T7 (MASMTGGQQMG)
MASMTGGQQMG (T7) conjugated to KLH |
| Application【应用】 |
Research Sub Category
Epitope Tags Anti-T7 Epitope Tag Antibody, T7 (MASMTGGQQMG) detects level of T7 Epitope Tag & has been published & validated for use in IP, WB & IC. Western blot: 1:1,000-1:10,000 colorimetric; 1:1,000-1:30,000 ECL. Immunocytochemistry: 1:100-1:400 ELISA: 1:100-1:500 for coating; 1:1,000-1:30,000 for primary. Immunoprecipitation: 2-5μg/mL in 300-500μL of cell lysate (150-300μg/mL total protein). Lysis buffer 0.5% triton X-100 with proteinase inhibitors. Use highquality protein A beads or anti-rabbit conjugated beads for best results. Run bead-only (no T7 antibody) controls to mark bands related to protein A breakdown products. Optimal working dilutions must be determined by the end user. Research Category Epitope Tags & General Use |
| Physical form【外形】 |
Affinity purified immunoglobulin. Liquid in PBS, pH 7.2 with 0.1% sodium azide.
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| Other Notes【其他说明】 |
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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| Legal Information【法律信息】 |
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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半岛bd体育手机客户端 性质
| biological source【生物来源】 |
rabbit
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| Quality Level【质量水平】 |
100
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| antibody form【抗体形式】 |
affinity purified immunoglobulin
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| clone【克隆】 |
polyclonal
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| purified by【纯化方式】 |
affinity chromatography
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| species reactivity (predicted by homology) |
all
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| manufacturer/tradename |
Chemicon
?
|
| technique(s) |
immunocytochemistry: suitable
immunoprecipitation (IP): suitable western blot: suitable |
| shipped in【运输】 |
wet ice
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半岛bd体育手机客户端 说明
| Storage and Stability【储存及稳定性】 |
Maintain at 2-8°C in undiluted for up to 6 months from date of receipt.
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| Disclaimer【免责声明】 |
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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安全信息
| Storage Class Code【储存分类代码】 |
12 - Non Combustible Liquids
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| WGK |
WGK 2
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