Anti-IP3R3 Antibody, clone 6G12.1

32160702

InsP3R3, IP3 receptor isoform 3, IP3R 3, Type 3 inositol 1,4,5-trisphosphate receptor, Type 3 InsP3 receptor) is encoded by the ITPR3 gene (Gene ID 3710) in human. The inositol 1,4,5-trisphosphate (InsP3) receptors, InsP3R1, InsP3R2, and InsP3R3, play an important role in maintaining normal epithelial secretory function by mediating intracellular Ca2+ release. InsP3R3 is the major isoform in hepatic bile ducts, where it is concentrated along the subapical portion of the ER and regulates bicarbonate (HCO3 ) and chloride (Cl ) secretion into the bile. Wherease hepatocytes express mainly InsP3R2 that is involved in modulating the secretion of bile solutes. A loss of InsP3R3 expression in cholangiocytes is found in primary biliary cirrhosis (PBC), an autoimmune liver disease characterized by the loss of bile ducts and impaired bile secretion. Likewise, InsP3R3 and InsP3R2 double knockout mice are reported to exhibit severe defects of pancreatic zymogen secretion. Human InsP3R3 is a 6-transmembrane (a.a. 2203-2223, 2236-2256, 2265-2285, 2326-2346, 2369-2389, 2497-2517) ER membrane protein, having both its N- and C-terminal ends (a.a. 1-2202 and 2518-2671) exposed at the cytoplasmic side. The N-terminal cytoplasmic region contains five MIR (Mannosyltransferase, InsP3R, RyR) domains (a.a. 113-173, 174-224, 232-288, 295-372, 378-434) and the InsP3-binding sites (a.a. 266-270, 507-510, 567-569).

Clone 6G12.1 targets an epitope in the C-terminal half of human IP3R3 (IP3 Receptor 3) N-terminal cytoplasmic domain.

GST-tagged recombinant human IP3R3 (IP3 Receptor 3) N-terminal cytoplasmic domain fragment.

Anti-IP3R3 Antibody, clone 6G12.1, Cat. No. MABN1544, is a highly specific mouse monoclonal antibody that targets ITPR3 and has been tested in Western Blotting.
Research Category
Neuroscience

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IP3R3 (IP3 Receptor 3) in 10 ?g of HeLa cell lysate.

Protein G purified.
Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Concentration: Please refer to lot specific datasheet.

100

mouse

purified antibody

primary antibodies

6G12.1, monoclonal

human

western blot: suitable

IgG2bκ

NP_002215

Q14573

ambient

~260 kDa observed. 304.1 kDa calculated.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1