Anti-Interferon-β Antibody

32160702

NA.41

Interferon beta is a globular protein consisting of 5 alpha helices with a predicted molecular weight of 20 kDa. Due to glycosylation, however, the observed molecular weight is closer to 25 kDa by SDS-PAGE.

Recognizes Human interferon beta.

Recombinant human interferon beta expressed in E. coli

Research Sub Category
Cytokines & Cytokine Receptors
This Anti-Interferon-β Antibody is validated for use in ELISA, IH, IP, NEUT, WB for the detection of Interferon-β.
Interferon neutralization: 2 x 10e5 neutralization units/mL where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon beta (Hu-IFN-b) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," in J. Virol., Vol. 37, 755-758]; [Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods of Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon beta provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for the effective neutralization of human interferon beta.

Immunohistochemistry

Western blot: 1:5000

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology

Replaces: 04-1095

Unpurified
Rabbit Serum diluited with PBS. Liquid containing no preservatives.

Control
IFN expressing cell lines

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

rabbit

100

serum

primary antibodies

polyclonal

human

Chemicon ^?

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable

NM_000600.1

P05231

dry ice

20 kDa

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2