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Anti-Interferon-β Antibody

半岛bd体育手机客户端 编号: 4154528
规格: serum, Chemicon?
包装规格: 100 μL
半岛bd体育手机客户端 类别: 进口试剂
品牌: Sigma-Aldrich
优惠价: 立即咨询
半岛bd体育手机客户端 价格
半岛bd体育手机客户端 编号 包装单位 单价(元) 国内现货 国外库存 询价单
4154528 100 μL 7120
基本信息
eCl@ss
32160702
NACRES
NA.41
Specificity【特异性】
Reacts with mouse Interferon beta (IFNbeta). No reactivity is seen with IFNalpha or gamma
Immunogen【免疫原】
Mouse IgG, whole molecule.
Application【应用】
Research Sub Category
Cytokines & Cytokine Receptors
Detect Interferon-β using this Anti-Interferon-β Antibody validated for use in ELISA, IP, WB, IH, NEUT.
Cytopathic effect inhibition assay: 2 x 10E5 neutralization units per mL of antiserum. One neutralization unit is the amount of antiserum required to neutralize 1 unit of mouse IFN-beta. This material is prepared specifically for effective neutralization of mouse IFN-beta.

Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:

1. Put protein (approx 50ng) in blocking solution for 30-60 min RT

2. Add Primary antibody (1:500) to blocking solution

3. Agitate membrane for 60 min, RT

4. Wash 3X for 5min each with PBS-Tween

5. Add secondary antibody (1:1000) after diluting in PBS-Tween

6. Gentle agitation for 60min.

7. Wash 3X for 5min each with PBS-Tween

8. Develop with reaction kit of choice

Immunoprecipitation

Immunohistochemistry

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Physical form【外形】
Rabbit polyclonal serum in buffer containing 0.3 M NaHCO3 and 200 mM NaCl.
Legal Information【法律信息】
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
biological source【生物来源】
rabbit
Quality Level【质量水平】
100
antibody form【抗体形式】
serum
antibody product type
primary antibodies
clone【克隆】
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon ?
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
NCBI accession no.【NCBI登记号】
NM_002176.2
UniProt accession no.【UniProt登记号】
P01574
shipped in【运输】
dry ice
packaging【包装】
100 μL in 2x10(e4) units
2x10(e4) units
半岛bd体育手机客户端 说明
Storage and Stability【储存及稳定性】
Maintain frozen at -20°C or below for retention of full activity, for up to 12 months. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 1
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