FilBridge ELISA试剂盒

NA.32

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence of human GCSF. Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three dimensional structure of the protein and a basal level of epitopes are detected by this assay. The result is a histogram which can be likened to a ‘fingerprint′ for correctly folded Filgrastim. For an Filgrastim Biosimilar, if the protein is correctly folded and modified, the ‘fingerprint′ will match that of Filgrastim. If it is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the antibody made to that exposed sequence. In this way, changes in the ‘fingerprint′ generated by the ELISA will point out differences between the Biosimilar and authentic Filgrastim.

For research use only. Not for use in diagnostic procedures.

IgG fraction of antiserum

human (Human IgG)

wet ice

2-8℃

GHS05

Warning

H290 - H315 - H319

P305 + P351 + P338

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

8A - Combustible, corrosive hazardous materials