ADAM12 human

NA.32

A Disintegrin And Metalloprotease 12 (ADAM12) is also known as Meltrin-α and MCMP, is a member of the ADAM and MDC (Metalloprotease, Disintegrin, Cysteine-rich) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE. ADAM12 has four Isoform, Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. ADAM12 Involved in skeletal muscle regeneration, specifically at the onset of cell fusion and also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 month. For extended storage, it is recommended to store at -20℃.

human

expressed in HEK 293 cells

6-His tagged (C-terminus)

≥95% (SDS-PAGE)

lyophilized powder

calculated mol wt 34 kDa (Mature)
calculated mol wt 55.2 kDa
observed mol wt 23 kDa (DTT-reduced. Protein migrates due to glycosylation corresponding to the propeptide, the mature form and the pro form respectively. Leu24 is the predicted N-terminus.)
observed mol wt 46 kDa
observed mol wt 65 kDa

pkg of 10 μg

<1 EU/μg endotoxin (LAL test)

ADAM12

O43184

wet ice

?20℃

human ... ADAM12(8038)

11 - Combustible Solids

WGK 3