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Anti-Glucocorticoid Receptor (Ab-2) Mouse mAb (BuGR2)
| 半岛bd体育手机客户端 编号： | 4094379 |
| 规格： | lyophilized, clone BuGR2, Calbiochem? |
| 包装规格： | 100 μG |
| 半岛bd体育手机客户端 类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
半岛bd体育手机客户端 性质
| Quality Level【质量水平】 |
100
|
| biological source【生物来源】 |
mouse
|
| antibody form【抗体形式】 |
purified antibody
|
| antibody product type |
primary antibodies
|
| clone【克隆】 |
BuGR2, monoclonal
|
| form【形式】 |
lyophilized
|
| contains【包含】 |
≤0.1% sodium azide as preservative
|
| species reactivity |
rat, mouse, guinea pig, sheep, rabbit
|
| should not react with |
avian, amphibian, primate
|
| manufacturer/tradename |
Calbiochem
?
|
| storage condition【储存条件】 |
OK to freeze
|
| isotype【同位素/亚型】 |
IgG2a
|
| shipped in【运输】 |
ambient
|
| storage temp.【储存温度】 |
?20℃
|
| packaging【包装】 |
100 μg in Plastic ampoule
|
基本信息
| General description【一般描述】 |
Recognizes the ~94-97 kDa glucocorticoid receptor in mouse liver tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-AgX-653 myeloma cells (see application references). Recognizes the ~94-97 kDa glucocorticoid receptor. Anti-Glucocorticoid Receptor (Ab-2), mouse monoclonal, clone BuGR2, recognizes ~94-97 kDa glucocorticoid receptor in mouse liver. It is validated for WB, ICC, IP & IHC (Frozen & paraffin sections). |
| Immunogen【免疫原】 |
Rat
partially purified, rat glucocorticoid receptor |
| Application【应用】 |
Frozen Sections (5 g/ml) Immunoblotting (5 g/ml) Immunocytochemistry (5 g/ml) Immunoprecipitation (see comments) Paraffin Sections (5 g/ml) |
| Warning【警告】 |
Toxicity: Standard Handling (A)
|
| Physical form【外形】 |
Lyophilized from PBS, pH 7.2.
|
| Reconstitution【重悬】 |
Resuspend the lyophilized antibody with 0.1 ml sterile PBS, pH 7.2. Be careful to reconstitute the entire contents of the vial; during shipment and handling, portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
|
| Analysis Note【分析说明】 |
Positive Control
mouse liver extract |
| Other Notes【其他说明】 |
Does not cross react with androgen, progesterone, estrogen or mineralocorticoid receptors. Enzymatic digestion and immunoblot analysis has shown that this antibody reacts with the undigested 97 kDa glucocorticoid receptor, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid and DNA-binding chymotrypsin fragment. Immunocytochemical localization of the glucocorticoid receptor is observed in the cytoplasm and nucleus, even in the presence of hormone. This antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Hussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94,1521. Pratt, W.B. and Toft, D.O., 1997. Endocrine Rev. 18306. Schumacher, M., et al. 1996. Dev. Neurosci. 18,6. Toran-Allerand, C.D. 1996. Dev. Neurosci. 18,36. Weigel, N.L. 1996. Biochem J. 319,657. Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743,141. Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), 82. |
| Legal Information【法律信息】 |
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
|
安全信息
| Storage Class Code【储存分类代码】 |
11 - Combustible Solids
|
| WGK |
WGK 1
|
