Anti-IGF-IRα subunit Antibody

32160702

NA.41

Insulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor has tyrosine kinase that is activated by IGF-1 and IGF-2 binding. IGF-1 receptor is initially produced as a precursor protein with an aignal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine auto-phosphorylation of the beta chains, which in turn triggers cell type-specific downstream signaling cascade of events.

Epitope: Third Fibronectin Type III (FnIII 3) domain
KLH-conjugated synthetic peptide [C-KYADGTIDIEEVTENPKT] corresponding to amino acid residues 672-689 of human IGF-IR alpha chain.

Immunoprecipitation Analysis: 5.0 ?g from a representative lot immunoprecipitated human IGF-IRα subunit in 0.5 mg of human IGF-IR-expressing C4 cell lysate.
Immunocytochemistry Analysis: 2.0 ?g/mL from a representative lot detected IGF-IRα subunit in human IGF-IR-expressing C4 cells.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
This Anti-IGF-IRα subunit Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of IGF-IRα subunit.

Evaluated by Western Blotting in human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Western Blotting Analysis: 0.2 ?g/mL of this antibody detected IGF-IRα subunit in 10 ?g of human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Affinity purified
Purified chicken polyclonal IgY antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Concentration: Please refer to lot specific datasheet.

chicken

100

purified immunoglobulin

primary antibodies

polyclonal

affinity chromatography

human

rhesus macaque (based on 100% sequence homology)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

IgY

NP_000866

P08069

wet ice

human ... IGF1R(3480)

~110/180 kDa observed

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1