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Anti-Integrin β1 Antibody, clone N29 (Azide Free)
| 半岛bd体育手机客户端 编号： | 4093965 |
| 规格： | clone N29, Chemicon?, from mouse |
| 包装规格： | 100 μG |
| 半岛bd体育手机客户端 类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
半岛bd体育手机客户端 别名
Anti-Integrin β1 Antibody, clone N29 (Azide Free)
CD29, MAB2252
基本信息
| eCl@ss |
32160702
|
| NACRES |
NA.41
|
| Specificity【特异性】 |
Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).
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| Immunogen【免疫原】 |
Jurkat T-leukemic cell line
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| Application【应用】 |
Research Category
Cell Structure This Anti-Integrin β1 Antibody, clone N29 (Azide Free) is validated for use in Flow Cytometry and Immunohistochemistry and Immunoprecipitation and Affects Function and Cell Culture and Western Blotting for the detection of Integrin β1. Research Sub Category Integrins Immunohistochemistry (Paraffin) Analysis:A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue. Immunoprecipitation:A representative lot of this antibody clone was used in immunoprecipitation. Immunohistochemistry:A representative lot of this antibody clone was used in immunohistochemistry on frozen sections. Flow Cytometry:A representative lot of this antibody clone was used in flow cytometry. Functional Activity Assay:A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996). Optimal working dilutions must be determined by end user. |
| Quality【质量】 |
Western Blot Analysis:0.5 ?g/mL of the antibody detected Integrin β1 in 10 ?g of U251 cell lysate.
|
| Physical form【外形】 |
Format: Purified
Protein A purified Purified cultured supernatant in PBS without preservatives. |
| Analysis Note【分析说明】 |
Control
Tonsil, human skin, human kidney tissue U251 cell lysate |
| Other Notes【其他说明】 |
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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| Legal Information【法律信息】 |
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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半岛bd体育手机客户端 性质
| Quality Level【质量水平】 |
100
|
| biological source【生物来源】 |
mouse
|
| antibody form【抗体形式】 |
culture supernatant
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| antibody product type |
primary antibodies
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| clone【克隆】 |
N29, monoclonal
|
| species reactivity |
human
|
| manufacturer/tradename |
Chemicon
?
|
| technique(s) |
cell culture | mammalian: suitable
flow cytometry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| isotype【同位素/亚型】 |
IgGκ
|
| NCBI accession no.【NCBI登记号】 |
NM_002292.3
|
| UniProt accession no.【UniProt登记号】 |
P55268
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| shipped in【运输】 |
wet ice
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半岛bd体育手机客户端 说明
| Target description【目标描述】 |
88 kDa
|
| Storage and Stability【储存及稳定性】 |
Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles. |
| Disclaimer【免责声明】 |
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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安全信息
| Storage Class Code【储存分类代码】 |
12 - Non Combustible Liquids
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| WGK |
WGK 1
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