Anti-Recoverin Antibody

32160702

NA.41

Recoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. Recoverin is initially produced as a 200-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. Recoverin is composed of four EF-hand domains (a.a. 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. In photoreceptor cells, recoverin functions as a Ca2+-dependent inhibitor of G-protein-coupled receptor kinase 1 (GRK1). Recoverin plays an important role in mediating calcium-dependent rod and cone light adaptation. Recoverin delays the rod & cone response under dim light when [Ca2+] is high by inhibiting GRK1-catalyzed visual pigment phosphorylation, but has little effect during light adaptation when [Ca2+]i is lowered.

KLH-conjugated linear peptide corresponding to a sequence from the EF-hand 4 domain of human recoverin.
Epitope: EF-hand 4

Anti-Recoverin Antibody detects level of Recoverin and has been published and validated for use in Western Blotting, Immunohistochemistry.
Research Sub Category
Sensory & PNS
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human and rat retina tissue sections.
Research Category
Neuroscience

Evaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 0.25 ?g/mL of this antibody detected Recoverin in 10 ?g of human retina tissue lysate.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

Concentration: Please refer to lot specific datasheet.

rabbit

100

affinity isolated antibody

primary antibodies

polyclonal

affinity chromatography

human, rat

mouse (based on 100% sequence homology)

immunohistochemistry: suitable
western blot: suitable

NP_002894

P35243

wet ice

~23 kDa observed.

Stable for 1 year at 2-8°C from date of receipt.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1