您的位置：首页 > 半岛bd体育手机客户端 中心 > Anti-GSK3α/β Antibody, rabbit monoclonal
Anti-GSK3α/β Antibody, rabbit monoclonal
| 半岛bd体育手机客户端 编号： | 4082493 |
| 规格： | culture supernatant, from rabbit |
| 包装规格： | 100 μl |
| 半岛bd体育手机客户端 类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
半岛bd体育手机客户端 别名
Anti-GSK3α/β Antibody, rabbit monoclonal
GSK-3 alpha, glycogen synthase kinase 3 alpha
基本信息
| eCl@ss |
32160702
|
| NACRES |
NA.41
|
| General description【一般描述】 |
Glycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer′s disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
|
| Specificity【特异性】 |
Predicted to cross-react with rat based on sequence homology.
GSK3 alpha and beta. |
| Immunogen【免疫原】 |
N-terminal 6His-tagged recombinant full length human GSK3β.
|
| Application【应用】 |
Research Category
Signaling Research Sub Category PI3K, Akt, & mTOR Signaling Immunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate. NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release This Anti-GSK3α/β Antibody is validated for use in WB, IP for the detection of GSK3α/β. |
| Quality【质量】 |
Evaluated by western blot on lysates from HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells. |
| Linkage【联系】 |
Replaces: 05-903
|
| Physical form【外形】 |
Cultured supernantant containing 0.05% sodium azide.
|
| Analysis Note【分析说明】 |
Control
HEK293 cell lysate |
| Other Notes【其他说明】 |
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
|
半岛bd体育手机客户端 性质
| biological source【生物来源】 |
rabbit
|
| Quality Level【质量水平】 |
100
|
| antibody form【抗体形式】 |
culture supernatant
|
| antibody product type |
primary antibodies
|
| clone【克隆】 |
monoclonal
|
| species reactivity |
rat, human, mouse
|
| technique(s) |
immunoprecipitation (IP): suitable
western blot: suitable |
| isotype【同位素/亚型】 |
IgG
|
| NCBI accession no.【NCBI登记号】 |
NP_002084.2
NP_063937.2 |
| UniProt accession no.【UniProt登记号】 |
P49840
|
| shipped in【运输】 |
dry ice
|
半岛bd体育手机客户端 说明
| Target description【目标描述】 |
~46 kDa (beta) and 51 kDa (alpha)
|
| Storage and Stability【储存及稳定性】 |
Stable for 1 year at -20?C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Disclaimer【免责声明】 |
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
安全信息
| Storage Class Code【储存分类代码】 |
12 - Non Combustible Liquids
|
| WGK |
WGK 1
|
