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Anti-VP16 antibody produced in rabbit
半岛bd体育手机客户端 编号: | 3405115 |
规格: | IgG fraction of antiserum, buffered aqueous solution |
包装规格: | 200 μL |
半岛bd体育手机客户端 类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
基本信息
MDL number | MFCD02264216 |
NACRES | NA.56 |
General description【一般描述】 | Anti-VP16 is produced in rabbit using a synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue. Whole antiserum is fractionated and further purified by ionexchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. |
Immunogen【免疫原】 | synthetic peptide corresponding to amino acids 474-487 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine residue. |
Application【应用】 | Anti-VP16 recognizes VP16 fusion proteins by immunoblotting and immunoprecipitation. It is used to study the effect of sialic acid on herpes simplex virus type 1 envelope glycoproteins. It is also used to study if self-association of lymphocytic choriomeningitis virus nucleoprotein is mediated by its N-terminal region and is not required for its anti-interferon function. |
Physical form【外形】 | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
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性质
biological source【生物来源】 | rabbit |
Quality Level【质量水平】 | 200 |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | IgG fraction of antiserum |
antibody product type | primary antibodies |
clone【克隆】 | polyclonal |
form【形式】 | buffered aqueous solution |
technique(s) | immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
安全信息
Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
WGK | WGK 3 |