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半岛bd体育手机客户端
性质
| biological source【生物来源】 | human muscle |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Near Diploid |
| morphology【形态学】 | Epithelial-like |
| products【半岛bd体育手机客户端 】 | Transforming growth factor (Tramnsforming Growth Factor (TGF)) - ß |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human Ewing sarcoma |
| Cell Line Description【细胞系描述】 | Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice. The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status. |
| Application【应用】 | Tumourigenicity studies |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 11,12 D13S317: 8,13 D16S539: 11 D5S818: 11,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 15,18 |
| Culture Medium【培养基】 | DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
