EZview^TMRed Glutathione Affinity Gel

MFCD05665380

NA.56

EZview? Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer.

The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview ^?Red Affinity Gels greatly reduce the risk of pellet loss. EZview ^?resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

EZview is a trademark of sigma-aldrich Co. LLC

200

proteins (GST)

immunoprecipitation (IP): suitable
protein purification: suitable

2-8℃

1 mL in poly bottle

10 - Combustible liquids

WGK 3