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常见荧光染料峰激发波长和发射波长
2024.09.23 点击4122次

荧光染料通常溶于有机溶剂或水溶液中。不同溶剂的极性不同,染料分子在不同溶剂中的环境也不同,导致荧光发射峰的偏移的情况发生。因此建议使用前咨询染料制造商来确认特定荧光染料的数据,以确保激发和发射曲线的完整。

荧光染料 激发波长 发射波长
3-Hydroxypyrene-5,-8,10-TriSulfonic Acid 403 513
5-(and 6-)carboxy SNARF-1 indicator 548(low pH) 587(low pH)
5-(and 6-)carboxy SNARF-1 indicator 576(high pH) 635(high pH)
5-Hydroxy-Tryptamine (5-HT) 380-415 520-530
6-Carboxyrhodamine 6G 525 555
7-Aminoactinomycin D (7-AAD) ? 546 647
7-Hydroxy-4-methylcoumarin 360 455
Acid Fuchsin 540 630
ACMA 430 474
Acridine orange 500 (+DNA) 526 (+DNA)
Acridine orange 460 (+RNA) 650 (+RNA)
Acridine Orange (Bound to DNA) 502 526
Acridine Red 455-600 560-680
Acridine Yellow 470 550
Acriflavin 436 520
AFA (Acriflavin Feulgen SITSA) 355-425 460
Alexa Fluor 350 dye, 7-amino-4-methylcoumarin (AMC) 346 442
Alexa Fluor 405 dye 402 421
Alexa Fluor 430 dye 434 539
Alexa Fluor 488 dye 495 519
Alexa Fluor 514 dye 518 540
Alexa Fluor 532 dye 531 554
Alexa Fluor 546 dye 556 573
Alexa Fluor 555 dye 555 565
Alexa Fluor 568 dye 578 603
Alexa Fluor 594 dye 590 617
Alexa Fluor 610 dye 612 628
Alexa Fluor 633 dye 632 647
Alexa Fluor 635 dye 633 647
Alexa Fluor 647 dye 650 668
Alexa Fluor 660 dye 663 690
Alexa Fluor 680 dye 679 702
Alexa Fluor 700 dye 702 723
Alexa Fluor 750 dye 749 775
Alexa Fluor 790 dye 782 805
Alizarin Complexon 530-560 580
Alizarin Red 530-560 580
Allophycocyanin 650 660
AMCA-S, AMC 345 445
Aminoactinomycin D 555 655
Aminocoumarin 350 445
Anthroyl Stearate 361-381 446
Astrazon Brilliant Red 4G 500 585
Astrazon Orange R 470 540
Astrazon Red 6B 520 595
Astrazon Yellow 7 GLL 450 480
Atabrine 436 490
Auramine 460 550
Aurophosphine 450-490 515
Aurophosphine G 450 580
BAO 9-(Bisamino-phenyloxadiazole) 365 395
BCECF 505 530
BCECF indicator 482 (low pH) 520 (low pH)
BCECF indicator 503 (high pH) 528 (high pH)
Berberine Sulphate 430 550
Bimane 380 458
Bisbenzamide 360 600-610
Blancophor FFG Solution 390 470
Blancophor SV 370 435
BOBO-1, BO-PRO-1 462 481
BODIPY 630/650 dye 630 650
BODIPY 650/665 dye 650 665
BODIPY FL dye 505 513
BODIPY TMR-X dye 542 574
BODIPY TR-X dye 589 617
BOPRO 1 462 481
Brilliant Sulpho-flavin FF 430 520
Calcein 494 517
Calcien Blue 370 435
Calcium Crimson indicator 590 615
Calcium Green indicators 506 533
Calcium Orange indicator 549 576
Calcofluor RW Solution 370 440
Calcofluor White 440 500-520
Calcofluor White ABT Solution 380 475
Calcofluor White Standard Solution 365 435
Carboxy SNARF indicators 548 (low pH) 587 (low pH)
Carboxy SNARF indicators 576 (high pH) 635 (high pH)
Cascade Blue dye 400 420
Cascade Yellow dye 402 545
Catecholamine 410 470
Chinacrine 450-490 515
CL-NERF 504(low pH) 587(low pH)
CL-NERF 514(high pH) 540(high pH)
Coriphosphine O 460 575
Coumarin-Phalloidin 387 470
CY3.18 554 568
CY5.18 649 666
CY7 710 805
DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid) 340 525
DANSA (DiaminoNaphthyl-Sulphonic Acid) 340-380 430
Dansyl NH-CH3 in water 340 578
DAPI ? 358 461
Di-8-ANEPPS 488 605
Di-8-ANEPPS, Di-4-ANEPPS ? 468 635
DiA ? 456 590
Diamino Phenyl Oxydiazole (DAO) 280 460
DiD (DiIC18(5)) 644 665
DiI (DiIC18(3)) 549 565
Dimethylamino-5-Sulphonic Acid 310-370 520
DiO (DiOC18(3)) 484 501
Diphenyl Brilliant Flavine 7GFF 430 520
DiR (DiIC18(7)) 750 779
DM-NERF 497(low pH) 527(low pH)
DM-NERF 510(high pH) 536(high pH)
Dopamine 340 490-520
ELF 97 alcohol 345 530
Eosin 524 544
ER Tracker Blue-White DPX ? 372 556
Erythrosin ITC 530 558
EthD-1 ? 528 617
Ethidium bromide ? 518 605
Euchrysin 430 540
FIF (Formaldehyde Induced Fluorescence) 405 435
Flazo Orange 375-530 612
Fluo-3 indicator 506 526
Fluo-4 indicator 494 516
Fluorescein 494 518
Fluorescein Iso-thiocyanate (FITC) 490 525
Fluorescein, FITC 494 518
FM 1-43, FM 1-43FX ? 479 598
FM 4-64, FM 4-64FX ? 506 750
FM1-43 479 598
Fura Red indicator 472 (low [Ca2+]) 657 (low [Ca2+])
Fura Red indicator 436 (high [Ca2+]) 637 (high [Ca2+])
Fura-2 indicator 363 (low [Ca2+]) 512 (low [Ca2+])
Fura-2 indicator 335 (high [Ca2+]) 505 (high [Ca2+])
Genacryl Brilliant Red B 520 590
Genacryl Brilliant Yellow 10GF 430 485
Genacryl Pink 3G 470 583
Genacryl Yellow 5GF 430 475
Gloxalic Acid 405 460
Granular Blue 355 425
Haematoporphyrin 530-560 580
Hoechst 33258, Hoechst 33342 ? 352 461
Indo-1 indicator 346 (low [Ca2+]) 475 (low [Ca2+])
Indo-1 indicator 330 (high [Ca2+]) 401 (high [Ca2+])
Intrawhite Cf Liquid 360 430
JC-1 498 (monomer) 525 (monomer)
JC-1 593 (aggregate) 595 (aggregate)
JC-9 506 525 (monomer)
JC-9 506 635 (aggregate)
JOE dye 525 555
Leucophor PAF 370 430
Leucophor SF 380 465
Leucophor WS 395 465
Lissamine rhodamine B 570 590
Lissamine Rhodamine B200 (RD200) 575 595
Lucifer yellow CH 428 536
Lucifer Yellow VS 430 535
LysoSensor Blue DND-167 374 425
LysoSensor Blue DND-192, DND-167 374 425
LysoSensor Green DND-153, DND-189 442 505
LysoSensor Yellow/Blue DND-160 (PDMPO) 384 (low pH) 540 (low pH)
LysoSensor Yellow/Blue DND-160 (PDMPO) 329 (high pH) 440 (high pH)
LysoTracker Green 504 511
LysoTracker Red 577 592
LysoTracker Yellow 534 551
Magdala Red 524 600
Magnesium Green indicator 506 531
Magnesium Orange 550 575
Marina Blue dye 365 460
Maxilon Brilliant Flavin 10 GFF 450 495
Maxilon Brilliant Flavin 8 GFF 460 495
Mithramycin 450 570
MitoTracker Green FM 490 516
MitoTracker Orange CMTMRos 551 576
MitoTracker Red CMXRos 578 599
Monobromobimane § 394 490
MPS (Methyl Green Pyronine Stilbene) 364 395
NBD 465 535
NBD amines 465 535
NeuroTrace 500/525 green-fluorescent Nissl stain ? 500 525
Nile red ? 549 628
Nitrobenzoxadidole 460-470 510-650
Noradrenaline 340 490-520
Nuclear Fast Red 289-530 580
Nuclear Yellow 365 495
Nylosan Brilliant Flavin E8G 460 510
Oregon Green 488 dye and Oregon Green 488 BAPTA indicators 496 524
Oregon Green 488 fluorophore 496 524
Oregon Green 500 fluorophore 503 522
Oregon Green 514 dye 511 530
Oregon Green 514 fluorophore 511 530
Pacific Blue dye 410 455
Pacific Orange dye 400 551
Pararosaniline (Feulgen) 570 625
Phorwite AR Solution 360 430
Phorwite BKL 370 430
Phorwite Rev 380 430
Phorwite RPA 375 430
Phosphine 3R 465 565
Phosphine R 480-565 578
Pontochrome Blue Black 535-553 605
POPO-1, PO-PRO-1 ? 434 456
Primuline 410 550
Procion Yellow 470 600
Propidium iodide ? 536 617
Pyronine 410 540
Pyronine B 540-590 560-650
Pyrozal Brilliant Flavin 7GF 365 495
Quinacrine Mustard 423 503
Resorufin 571 585
RH 414 ? 500 635
Rhod-2 indicator 552 578
Rhodamine 110 496 520
Rhodamine 123 507 529
Rhodamine 5 GLD 470 565
Rhodamine 6G 526 555
Rhodamine B 540 625
Rhodamine B 200 523-557 595
Rhodamine B Extra 550 605
Rhodamine BB 540 580
Rhodamine BG 540 572
Rhodamine Green dye 502 527
Rhodamine Red dye 570 590
Rhodamine WT 530 555
Rhodol Green fluorophore 499 525
Rose Bengal 540 550-600
ROX dye 580 605
R-phycoerythrin 565 575
Serotonin 365 520-540
Sevron Brilliant Red 2B 520 595
Sevron Brilliant Red 4G 500 583
Sevron Brilliant Red B 530 590
Sevron Orange 440 530
Sevron Yellow L 430 490
SITS (Primuline) 395-425 450
SITS (Stilbene Isothiosulphonic Acid) 365 460
Snarf 1 563 639
Sodium Green indicator 507 535
Stilbene 335 440
Sulpho Rhodamine B Can C 520 595
Sulpho Rhodamine G Extra 470 570
SYTO 17 red fluorescent nucleic acid stain 621 634
SYTO blue-fluorescent nucleic acid stains 40, 41, 42, 43 ? 428 ± 6 454 ± 13
SYTO blue-fluorescent nucleic acid stains 44, 45 ? 450 ± 5 478 ± 7
SYTO green-fluorescent nucleic acid stains 11, 14, 15, 20, 22, 25 ? 515 ± 7 543 ± 13
SYTO green-fluorescent nucleic acid stains 12, 13, 16, 21, 23, 24 ? 494 ± 6 515 ± 7
SYTO orange-fluorescent nucleic acid stains 80, 81, 82, 83 ? 537 ± 7 552 ± 8
SYTO orange-fluorescent nucleic acid stains 84, 85 ? 567 583
SYTO red-fluorescent nucleic acid stains 17, 59, 61, 64 ? 615 ± 15 632 ± 13
SYTO red-fluorescent nucleic acid stains 60, 62, 63 ? 655 ± 3 675 ± 3
SYTOX Blue nucleic acid stain ? 445 470
SYTOX Green nucleic acid stain ? 504 523
SYTOX Orange nucleic acid stain ? 547 570
Tetracycline 390 560
Tetramethylrhodamine, Rhodamine B 555 580
Texas Red 596 615
Texas Red and Texas Red-X dye 595 615
Thiazine Red R 510 580
Thioflavin 5 430 550
Thioflavin S 430 550
Thioflavin TCN 350 460
Thiolyte 370-385 477-484
Thiozol Orange 453 480
Tinopol CBS 390 430
TOTO-1, TO-PRO-1 ? 514 533
TOTO-3, TO-PRO-3 ? 642 660
TRITC (Tetramethyl Rhodamine Isothiocyanate) 557 576
True Blue 365 420-430
Ultralite 656 678
Uranine B 420 520
Uvitex SFC 365 435
X-rhod-1 indicator 580 602
X-Rhodamine 580 605
XRITC 582 601
Xylene Orange 546 580
YOYO-1, YO-PRO-1 ? 491 509
YOYO-3, YO-PRO-3 ? 612 631
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