Atto 590 NHS ester

半岛bd体育手机客户端 说明

一般描述

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescencein situhybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.
Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes, which has an absorption maximum of 594 nm and an emission maximum of 624 nm. Atto 590-N-hydroxysuccinimide (NHS) is membrane permeable.

应用

Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, T_fl4.0 ns (unpublished data)
Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.

特点和优势

• Absorption maxima cover a range from 590 to 620 nm.
• Structural properties support extraordinary photostability.
• High fluorescence quantum yield and high thermal stability.
• The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
• Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

其他说明

Study of multistep energy transfer in a single photonic wire by FRET; employed in fluorescence energy transfer, FRET, scanning microscopy.

法律信息

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

基本信息

经验(实验)分子式	C41H42ClN3O11
分子量	788.24
MDL编号	MFCD03456133
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	100
半岛bd体育手机客户端 线	BioReagent
测定	≥60% (coupling to amines)
manufacturer/tradename	ATTO-TEC GmbH
荧光	λex590 nm; λem~620 nm in 0.1 M phosphate pH 7.0
适用性	suitable for fluorescence
detection method	fluorometric
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)