半岛bd体育手机客户端 说明
一般描述
Recombinant, human WEE1 fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed inS. frugiperdainsect cells using a baculovirus expression system. WEE1 is a tyrosine kinase that causes inhibitory phosporylation of Cdk1 and Cdk2 and acts as a mitotic inhibitor. Protects the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 prior to the onset of mitosis.
包装
10 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)6:2:5:1per min at 30°C, using variable concentrations of ATP (100 nM to 1.55 ?M).
外形
In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Katayama, K., et al. 2005.Mol. Cell. Biol.25,5725.
Yoshida, T., et al. 2004.Ann. Oncol.15,252.
Watanabe, N., et al. 1995.EMBO J.14,1878.
Igarashi, M., et al. 1991.Nature353,80.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 酶学委员会(EC)编号 | 2.7.1.112 ( BRENDA | IUBMB ) |
半岛bd体育手机客户端 性质
| 形式 | liquid |
| 质量水平 | 100 |
| specific activity | ≥1 units/mg protein |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 运输 | wet ice |
| 储存温度 | ?70℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
