Universal Protease Substrate

半岛bd体育手机客户端 说明

一般描述

The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present.

• Conveniently detect nanogram quantities of proteolytic activity in less than one hour.
• Perform highly sensitive protease detection in a homogeneous assay.

应用

Principle
By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.

Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay.

质量

Function test: performance-controlled in the assay for proteases.

制备说明

Working concentration:Approximately 1 mg/ml
Storage conditions (working solution):-15 to -25 ℃
An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 ℃ the product is rapidly hydrolyzed in solution.

其他说明

仅用于生命科学研究。不可用于诊断。

半岛bd体育手机客户端 性质

质量水平	100
形式	lyophilized
包装	pkg of 40 mg (40 μmol; 100mM)
manufacturer/tradename	Roche
浓度	1 mg/mL
application(s)	detection
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable