UKBi011-A

半岛bd体育手机客户端 说明

一般描述

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

细胞系来源

Depositor
Universita¨tsklinikum Bonn

细胞系描述

Derivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Vector map: File
Vector Free Reprogramming
Types of factor(s) used: mRNA

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60 Yes -
TRA 1-81 Yes -
hPSC Scorecard
Positive Negative
Self-renewal
Endoderm
Mesoderm
Ectoderm
Data analysis report: scorecard_mv.png
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative

Genotyping
Karyotyping
Passage number: 31
Cell line karyotype: normal
Karyotyping method: Molecular karyotyping by SNP array
Image / Result

联系

• Additional Cell Line Info
• EBiSC Access Use Agreement (EAUA)
• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

传代培养常规

Surface coating: Matrigel^?/Geltrex^?
Passage method: Enzyme-free cell dissociation / EDTA
O₂concentration: 21%
CO₂concentration: 6%
Culture medium: other
Culture base medium: StemMACS^?iPS-Brew XF
Main protein source: -
Passage Number banked (pre-EBiSC): 31
No. Vials banked (pre-EBiSC): 30

法律信息

EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
StemMACS is a trademark of Miltenyi Biotec GmbH

半岛bd体育手机客户端 性质

生物来源	human peripheral blood
重编程方法	Sendai virus
描述	age (80-84)
manufacturer/tradename	EBiSC?
性别	male
生长模式	adherent (pluripotent)
technique(s)	cell culture | stem cell: suitable
相关疾病	Alzheimer′s disease
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	6.2 - Infectious substances
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable