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位置: 首页> 品牌> Sigma-Aldrich> TSKgel?NH2-100 HPLC Column

TSKgel?NH2-100 HPLC Column

货号
821967
规格纯度
phase amine, L × I.D. 5 cm × 2 mm, 3 μm particle size
价格
14163.1 *本价格含增值税费
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半岛bd体育手机客户端 名称:
TSKgel ?NH2-100 HPLC Column
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

TSKgel NH2-100 amino columns expand the range of TSKgel columns for hydrophilic interaction chromatography (HILIC). Offering a different selectivity from the well known TSKgel Amide-80 columns, these novel amino-bonded phase columns stand out by providing much improved chemical stability, a prerequisite for achieving reproducible and reliable results. TSKgel NH2-100 columns are packed with 3 micron silica particles containing 100? pores. A novel bonding strategy was adopted to improve chemical stability of the bonded phase. First the silica is reacted with a trimethylsilane endcapping reagent at a low stoichiometric ratio, before reacting residual and accessible silanol groups with trifunctional alkylaminosilane reagent. The resulting bonded phase provides a better safeguard against hydrolysis of the underlying silica. TSKgel NH2-100 columns are unique in that the bonded phase ligand not only, as expected, has a terminal primary amino group, but that the spacer also incorporates secondary as well as tertiary amino groups. Anionic compounds are retained on the column by ionic interaction. This allows for the use of gradients in salt concentration, in addition to gradient elutions with acetonitrile. Since the TSKgel NH2-100 columns have cationic sites, the columns can be used as mixed mode columns under some conditions. TSKgel NH2-100 columns are well suited for the analysis of all types of hydrophilic compounds, including carbohydrates and peptides. Due to a high ligand density and large surface area, these columns show stronger retention of polar compounds than TSKgel Amide-80 columns.

外形

Shipped in 85% acetonitrile - 15% water.

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

基本信息

eCl@ss 32110501

半岛bd体育手机客户端 性质

物料 stainless steel hardware
agency suitable for USP L8
半岛bd体育手机客户端 线 TSKgel?
manufacturer/tradename Tosoh 21967
参数 0-100% organic solvent
0.2 mL/min standard flow rate
10-50 ℃ temperature
150 bar max. pressure
technique(s) HPLC: suitable
长度 × 内径 5 cm × 2 mm
基质 silica particle platform
基质活性基团 amine phase
粒径 3 μm
孔径 100 ?
表面积 450 m2/g
操作pH 2.0 - 7.5
分离技术 anion exchange
hydrophilic interaction (HILIC)
normal phase

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