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TSKgel?Chelate-5PW HPLC Column

货号
814440
规格纯度
phase iminodiacetic acid, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
价格
23187.9 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
TSKgel ?Chelate-5PW HPLC Column
TSKgel Chelate-5PW HPLC Column and Guardgel Kit
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

半岛bd体育手机客户端 性质

物料 glass
半岛bd体育手机客户端 线 TSKgel?
manufacturer/tradename Tosoh 14440
参数 ≤20% organic solvent
≤4 M salt concentration
≤45 ℃ temperature
1.0 mL/min flow rate
20 bar max. pressure
technique(s) HPLC: suitable
长度 × 内径 5 cm × 5 mm
基质 hydroxylated methacrylic polymer
基质活性基团 iminodiacetic acid phase
粒径 10 μm
操作pH 2 - 12
分离技术 affinity

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