TSKgel^?Chelate-5PW Guardgel Kit

半岛bd体育手机客户端 说明

一般描述

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

组分

TSKgel Guardgel kits contain:

• 5 mL Guardgel packing material
• Column holder, consisting of external reducing union, cap, frit, 2 ferrules
• Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"
• 2 Replacement frits
• Capillary tubing, 5 cm x 1/16" OD x 0.01"
• Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each
• All hardware is 316 stainless steel

Dimensions for Guardgel kits for 21.5 mm ID columns:

• Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
• Capillary tubing, 5 cm x 1/16" OD x 0.02" ID

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

半岛bd体育手机客户端 性质

物料	PEEK
半岛bd体育手机客户端 线	TSKgel?
manufacturer/tradename	Tosoh Chelate-5PW, 08647
标记范围	20 μmol/mL resin ligand density
参数	≤20% organic solvent ≤3 M salt concentration ≤45 ℃ temperature
technique(s)	LPLC: suitable
长度 × 内径	2.5 cm × 6 mm
基质	polymer particle platform
基质活性基团	iminodiacetic acid phase
粒径	20 μm
操作pH	2 - 12
相容性	mode of use affinity HPLC
分离技术	affinity