Trypsin, TPCK-Treated

半岛bd体育手机客户端 说明

一般描述

胰蛋白酶是一种丝氨酸蛋白酶，可特异性水解赖氨酸及精氨酸残基的羧基侧的多肽键。该款修饰后的胰蛋白酶经过甲苯磺酰基-L-氨基联苯氯甲基酮 (TPCK) 处理，可使外源性糜蛋白酶失活。每个包装含8个小瓶，每瓶含25 μg。

应用

Trypsin, TPCK-Treated has been used:

• as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) for porcine delta coronavirus (PDCoV) infection experiments using epithelial-like pig kidney cell line (LLC-PK1)
• to detach the human umbilical vein endothelial cells (HUVEC) for annexin-V/propidium Iodide (PI) staining assay
• in minimum essential medium (MEM) for multicycle replication kinetics

生化/生理作用

N-p-Tosyl- L -phenylalanine chloromethyl ketone (TPCK) serves as an irreversible inhibitor of chymotrypsin. Trypsin induces human fibrocyte differentiation. Trypsin is widely used in proteomics for protein sample digestion. In cell culture, trypsinization is carried out to dislodge adherent cells from each other and the walls of the culture vessel.

基本信息

EC 号	232-650-8
NACRES	NA.56

半岛bd体育手机客户端 性质

运输	dry ice
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H334 - H335
预防措施声明	P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官	Respiratory system
储存分类代码	10 - Combustible liquids
闪点(F)	Not applicable
闪点(C)	Not applicable