半岛bd体育手机客户端 说明
一般描述
Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.
包装
10 μg in Plastic ampoule
生化/生理作用
ED?? = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
警告
Toxicity: Standard Handling (A)
外形
Lyophilized from sterile-filtered PBS, 50 ?g BSA/?g TRAIL, pH 7.4.
重悬
Reconstitute to ≥20 ?g/ml in sterile PBS, 0.1% BSA.
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
其他说明
Griffith, T.S., et al. 1999.J. Exp. Med.189,1343.
Mariani, S.M., et al. 1997.J. Cell Biol.137,221.
Pan, G., et al. 1997.Science277,815.
Pan, G., et al. 1997.Science276,111.
Sheridan, J.P., et al. 1997.Science277,818.
Pitti, R.M., et al. 1996.J. Biol. Chem.271,12687.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 重组 | expressed in NSO cells |
| 测定 | ≥97% (SDS-PAGE) |
| 形式 | lyophilized |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze |
| 杂质 | ≤1 EU/μg Endotoxin (EU/μg TRAIL) |
| 运输 | ambient |
| 储存温度 | ?70℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
