SILu^TMProt TIMP2, Metalloproteinase inhibitor 2 human

半岛bd体育手机客户端 说明

一般描述

SILu^?Prot TIMP2 is a recombinant, stable isotope-labeled human TIMP2 which incorporates [¹³C₆,¹⁵N₄]-Arginine and^[13C₆,¹⁵N₂]-Lysine. Expressed in human 293 cells, it is designed to be used as an internal standard for bioanalysis of TIMP2 in mass-spectrometry. SILu^?Prot TIMP2 is a protein of 194 amino acids , with a calculated molecular mass of 21.7 kDa.

生化/生理作用

While the mammalian TIMP family has four members, TIMP-2 is a unique family member in that in addition to inhibiting matrix metalloproteinases (MMPs), TIMP-2 selectively interacts with MT1-MMP to facilitate the cell-surface activation of pro-MMP-2.1 Thus, TIMP-2 functions both as an inhibitor of MMPs, and is required for the cellular mechanism of pro-MMP-2 activation. Recently, it was validated that combination of TIMP-2 with another urinary cell-cycle arrest biomarkers, i.e. the insulin-like growth factor-binding protein 7 (IGFBP7) may predict the risk of moderate and severe acute kidney injury (AKI) in critically ill patients. For postoperative surgical intensive care unit patients, a single urinary TIMP2?IGFBP7 test accurately identified patients at risk for developing AKI within the ensuing 12 hours and its inclusion in clinical risk prediction models significantly enhances their performance.

序列

CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

外形

Supplied as a lyophilized powder containing phosphate buffered saline.

法律信息

SILu is a trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.12

半岛bd体育手机客户端 性质

重组	expressed in HEK 293 cells
质量水平	200
测定	≥98% (SDS-PAGE)
形式	lyophilized powder
效能	≥98% Heavy amino acids incorporation efficiency by MS
适用性	suitable for mass spectrometry (standard)
UniProt登记号	P16035
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable