SILu^TMProt SOST, Sclerostin human

半岛bd体育手机客户端 说明

一般描述

SILu^?Prot SOST is a recombinant, stable isotope-labeled human SOST which incorporates [¹³C₆,¹⁵N₄]-Arginine and [¹³C₆,¹⁵N₂]-Lysine. Expressed in human 293 cells, it is designed to be used as an internal standard for bioanalysis of SOST in mass-spectrometry. SILu^?Prot SOST is a protein of 201 amino acids (including a N-terminal polyhistidine and tag), with a calculated molecular mass of 22.8 kDa.

生化/生理作用

Sclerostin is a secreted Wnt signaling antagonist produced almost exclusively by osteocytes. It can selectively inhibit Wnt/ β-catenin, suppressing the activity of osteoblasts as well as the viability of osteoblasts and osteocytes. Lower sclerostin levels are associated with lower bone mineral content and bone. It was demonstrated that greater total limb bone mineral content was significantly associated with greater circulating levels of sclerostin. In addition, circulating sclerostin is a biomarker of osteoporosis severity in long-term, chronic paraplegia. Serum sclerostin was associated significantly, independently, and positively with bone mineral density of both cortical and cancellous bone. Sclerostin is considered to be one of the factors associated with chronic kidney disease-mineral and bone disorder in hemodialysis patients.

序列

HHHHHHHHGGQQGWQAFKNDATEIIPELGEYPEPPPELENNKTMNRAENGGRPPHHPFETKDVSEYSCRELHFTRYVTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRGKWWRPSGPDFRCIPDRYRAQRVQLLCPGGEAPRARKVRLVASCKCKRLTRFHNQSELKDFGTEAARPQKGRKPRPRARSAKANQAELENAY

外形

Supplied as a lyophilized powder containing phosphate buffered saline.

法律信息

SILu is a trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.12

半岛bd体育手机客户端 性质

质量水平	200
生物来源	human
重组	expressed in HEK 293 cells
标签	8-His tagged
测定	≥98% (SDS-PAGE)
形式	lyophilized powder
效能	≥98% Heavy amino acids incorporation efficiency by MS
technique(s)	mass spectrometry (MS): suitable
适用性	suitable for mass spectrometry (standard)
UniProt登记号	Q9BQB4
储存温度	?20℃
Gene Information	human ... SOST(50964)

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable