半岛bd体育手机客户端 说明
一般描述
A cell-permeable steroidal compound that selectively inhibits SHIP1-, but not SHIP2-, catalyzed PI(3,4,5)P3-to-PI(3,4)P2 dephosphorylation (IC50= 10 ?M vs. >1 mM, respectively), while exhibiting little activity against the PTEN-catalyzed PI(3,4,5)P3-to-PI(4,5)P2 conversion (IC50>1 mM). 3ACin vivotreatment (2.292 umol/200 ?l/mouse/daily i.p. for 7 d) is reported to boost both peripheral blood granulocyte production (by 4- to 5-fold) and the GVHD- (graft-versus-host disease) suppressing Mac1+Gr1+MIR (myeloid immunoregulatory) cell population in lymphoid tissues (by 5.55- and 11.53-fold in spleen and lymph nodes, respectively), resulting in an impaired splenocyte allogeneic T cell response in MLR (mixed-leukocyte/lymphocyte reaction) assays (by ≥95%). Similarly, 3AC-treated (9.4 ?M; 24 h) primary human PBMCs greatly lose their ability in priming allogeneic T cell response in MLR assays (by 74.5%). In addition, a faster blood cell recovery is also observed in mice receiving 3AC (2.292 umol/200 ?l/mouse/daily i.p. for 7 d) after sub-lethal irradiation (550 Rads). Selectively inhibits the growth and survival of SHIP1-dependent human KG-1 and murine C1498 myelogenous leukemia lines (IC50= 11.5 ?M), but not the SHIP1-negative human CML K562 and osteosarcoma MG63 lines. SHIP2 Inhibitor, AS1938909 is also available (Cat. No. 565840).
A cell-permeable steroidal compound that selectively inhibits SHIP1′ polyphosphatase activity toward PI(3,4,5)P3 (IC50= 10 ?M), but not SHIP2 5′ or PTEN′ polyphosphatase activity toward PI(3,4,5)P3 (IC50>1 mM). Effectively inhibits SHIP1-mediated immune response bothin vitroandin vivo, as well as SHIP1-dependent cancer cell survival. SHIP2 Inhibitor, AS1938909 is also available (Cat. No. 565840).
包装
10 mg in Glass bottle
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Brooks, R., et al. 2010.J. Immunol.184,3582.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C27H49N |
| 分子量 | 387.68 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥98% (1H-NMR) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze |
| 颜色 | white |
| 溶解性 | ethanol: 100 mg/mL DMSO: insoluble |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
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