半岛bd体育手机客户端 说明
一般描述
Binding of Ca2+to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).
Binding of Ca2+leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.
包装
50 mg in Plastic ampoule
生化/生理作用
Primary Target
Calcium Probes
Product does not compete with ATP.
Reversible: no
Cell permeable: no
警告
Toxicity: Irritant (B)
重悬
Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.
其他说明
Komada, H., et al. 1989.Cell Struct. Funct.14,141.
Vijayaraghavan, S. and Hoskins, D. 1989.Cell Calcium10,241.
Arslan, P., et al. 1985.J. Biol. Chem.260,2719.
Bryant, D.T. 1985.Biochem. J.226,613.
Grynkiewicz, G., et al. 1985.J. Biol. Chem.260,3440.
Ashley R.H., et al. 1984.Biochem. J.219,149.
Tsien, R.Y., et al. 1982.J. Cell Biol.94,325.
Tsien, R.Y. 1980.Biochemistry19,2396.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C26H23N3O10· 4K |
| 分子量 | 693.87 |
| MDL编号 | MFCD00065487 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze protect from light |
| 颜色 | yellow |
| 溶解性 | water: 10 mg/mL |
| 荧光 | λex332 nm (high) λex354 nm (low) λem505 nm (high) λem510 nm (low) |
| 运输 | ambient |
| 储存温度 | ?20℃ |
| InChI | 1S/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4 |
| InChI key | XAGUNWDMROKIFJ-UHFFFAOYSA-J |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
