PTEN, His-Tag^?and S-Tag, Human Recombinant,S. frugiperda

半岛bd体育手机客户端 说明

一般描述

Full-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His?Tag and S?Tag sequences and expressed inS. frugiperdainsect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.

包装

10 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP? per min at 37°C.

外形

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Harrington, L.S., et al. 2005.Trends Biochem. Sci.30,35.
Osaki, M., et al. 2004.Apoptosis9,667.
Lennon, G., et al. 1996.Genomics33,151.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
测定	≥80% (SDS-PAGE)
形式	liquid
specific activity	≥75 units/mg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable