半岛bd体育手机客户端 说明
一般描述
Recombinant, bovine c-GMP-specific phosphodiesterase expressed inS. frugiperdainsect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583).
Recombinant, bovine c-GMP-specific phosphodiesterase expressed inS. frugiperdainsect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 ?l per lane is recommended. In immunoblotting, gives band at ~92 kDa.
包装
50 μL in Plastic ampoule
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
外形
In sodium phosphate buffer, 20% glycerol, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Gibson, L.C.D., et al. 2006.Eur. J. Pharmacol.538,39.
Webb, D.J., et al. 1999.Am. J. Cardiol.83,21C.
Turko, I.V., et al. 1998.J. Biol. Chem.273,6460.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
形式 | liquid |
质量水平 | 100 |
specific activity | ≥50,000 units/mL |
manufacturer/tradename | Calbiochem? |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
运输 | wet ice |
储存温度 | ?70℃ |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
Sigma-Aldrich