PhosphoDetect Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9)

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425in HT-1080 cells treated with TGF-β1.
Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425.
This PhosphoDetect Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer^423/425).

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser^423/425phosphorylation sites of Smad3, coupled to KLH.

包装

50 μL in Plastic ampoule

应用

Immunoblotting (1:1000)
Immunoprecipitation (1:50)

警告

Toxicity: Standard Handling (A)

外形

In 150 mM NaCl, 10 mM HEPES, 100 ?g/ml BSA, 50% glycerol pH 7.5.

其他说明

Attisano, L., et al. 2002.Science296,1646.
Moustakas, A., et al. 2001.J. Cell Sci.114,4359.
Wrana, J. 2000.Science23,1.
Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween^?-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine,xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	C25A9, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	human
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1