PCR Core Kit^PLUS

半岛bd体育手机客户端 说明

一般描述

The PCR Core Kit^PLUSsupplies all basic reagents to perform a polymerase chain reaction with the exception of template DNA and appropriate primers. The kit uses Taq DNA Polymerase as the polymerase. In addition UNG, heat-labile is supplied in the kit for efficient degradation of contaminating DNA from previous PCR amplifications.

应用

The PCR Core Kit^PLUSis the solution for laboratories that have to run PCRs with non-standard reaction conditions and also have to make sure that samples are not contaminated with DNA from earlier amplification reactions.

特点和优势

The PCR Core KitPLUS is fromThermus aquaticusBM , expressed inE. coli. This highly processive 5′ → 3′ DNA polymerase lacks 3′ → 5′ exonuclease activity. It is stable during prolonged elevated temperatures (+95℃). During PCR, it retains over 80% activity after 30 cycles (1 minute, +95℃; 1 minute, +37℃; 1 minute, +72℃). The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95kD.

• Reliable reproducible results:

High lot-to-lot consistency.

• Flexible:

Quickly set up the appropriate PCR conditions.

• Optimize Mg²⁺:

The buffers is supplied without MgCl ₂.

• Eliminate false-positive results:

Remove carryover contamination using Uracil-DNA Glycosylase.

包装

1 kit containing 6 components.

质量

Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

半岛bd体育手机客户端 规格

The PCR Core Kit^PLUScan reduce the risk of false-positive results by perventing PCR carryover contamination. Supplied Uracil-DNA Glycosylase (UNG) catalyzes the removal of uracil from single- and double-stranded DNA originating from the incorporation of dUTP during DNA replication or deamination of cytosine. The abasic sites are susceptible to cleavage by heat under alkaline conditions. Ribouracil residues in RNA and dUTP are not substrates for UNG. Using this kit can eliminate preexisting PCR products by at least a factor of 10⁷.

其他说明

仅用于生命科学研究。不可用于诊断。

法律信息

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

半岛bd体育手机客户端 性质

质量水平	100
用途	sufficient for ≤50 reactions
manufacturer/tradename	Roche
参数	72 ℃ optimum reaction temp.
最佳pH	~9.0 (20 ℃)
运输	dry ice
储存温度	?20℃

组份列表

组份不可单独销售

货号	组份
	Uracil-DNA Glycosylase, heat-labile 1 U/μl
	Taq DNA Polymerase 5 U/μl
	dNTP Stock Solution (10 mM of each dNTP)
	PCR Buffer with MgCl210x concentrated
	PCR Buffer without MgCl210x concentrated
	MgCl2Stock Solution 25 mM

安全信息

危险声明	H412
预防措施声明	P273 - P501
危险分类	Aquatic Chronic 3
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	does not flash
闪点(C)	does not flash