Osteopontin, His·Tag^?, Mouse, Recombinant, Mouse

半岛bd体育手机客户端 说明

一般描述

Recombinant, mouse osteopontin fused at the C-terminus to a His?Tag sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴(Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca²⁺-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.
Recombinant, mouse osteopontin fused at the C-terminus to a His?Tag sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴(Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.
M.W. 65,000 and 30,000 under reducing conditions.

包装

50 μg in Plastic ampoule

生化/生理作用

~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10? cells/well

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 0.2 ?m sterile-filtered PBS, 50 ?g BSA/?g rmOPN.

重悬

Reconstitute to ≥100 ?g/ml with sterile PBS.
Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.

其他说明

Xie, Y., et al. 2001.Kidney Int.60,1645.
Chiba, S., et al. 2000.Microbiol. Immunol.44,319.
Miyazaki, Y., et al. 1990.J. Biol. Chem.265,14432.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

测定	≥95% (SDS-PAGE)
质量水平	100
形式	lyophilized
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
杂质	≤100 pg/μg Endotoxins (pg/μg rmOPN)
运输	ambient
储存温度	?70℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable